Phcmvrluc control

Manufactured by Promega

The PhCMVRLuc control is a plasmid that contains the Renilla luciferase (RLuc) reporter gene under the control of the CMV promoter. It can be used as a positive control for gene expression assays utilizing the RLuc reporter.

Automatically generated - may contain errors

Lab products found in correlation

Luciferase assay reagent, by Biotium (2 mentions) Coelenterazine, by Biosynth (2 mentions) Lb953 luminometer, by Berthold Technologies (2 mentions) Pgl3 basic, by Promega (1 mentions)

2 protocols using phcmvrluc control

Luciferase Assay of TGIF1 Transcriptional Activity

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All luciferase reporter constructs were generated in pGL3 basic (Promega) by PCR from genomic DNA. Cells were transfected with firefly luciferase reporters and a phCMVRLuc control (Promega), with pCMV5 TGIF1 as indicated (2, 6, 18 ng per well), using PEI. The two mutant TGIF1 constructs encode TGIF1 with changes in conserved DNA-binding residues in the homeodomain: either Arg91 altered to methionine (R91M) or Asp88 altered to serine (N88S) (Bjerke et al. 2011 (link)). After 48 h, activity was assayed with luciferase assay reagent (Biotium) using a Berthold LB953 luminometer. Results were normalized using Renilla luciferase activity and assayed with 0.09 μM coelenterazine (Biosynth) as in Hyman-Walsh et al. (2010) (link). Results of replicate transfections are shown (N = 3; mean + standard deviation) normalized to the RLuc transfection control.

Shah A., Melhuish T.A., Fox T.E., Frierson HF J.r, & Wotton D. (2019). TGIF transcription factors repress acetyl CoA metabolic gene expression and promote intestinal tumor growth. Genes & Development, 33(7-8), 388-402.

+ Open protocol

+ Expand

YAP1-Dependent Luciferase Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were transfected with the 8×GTIIC-luc firefly luciferase reporter and a phCMVRLuc control (Promega, Madison, WI), with pcDNA Flag Yap1 as indicated, using PEI. After 48 hours, activity was assayed with luciferase assay reagent (Biotium) using a Berthold LB953 luminometer. Results were normalized using Renilla luciferase activity, assayed with 0.09μM coelenterazine (Biosynth, Naperville, IL), as in [51 (link)]. Results of replicate transfections are shown (N=3–6, mean plus standard deviation) normalized to the RLuc transfection control. The YAP1-luc reporter was a kind gift from Dr. R. Janknecht (University of Oklahoma) [52 (link)], and mutant versions were generated within this plasmid by PCR.

Melhuish T.A., Kowalczyk I., Manukyan A., Zhang Y., Shah A., Abounader R, & Wotton D. (2018). Myt1 and Myt1l transcription factors limit proliferation in GBM cells by repressing YAP1 expression.. Biochimica et biophysica acta. Gene regulatory mechanisms, 1861(11), 983-995.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!