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Mir 162

Manufactured by Sanyo
Sourced in Japan

The MIR-162 is a laboratory equipment product from Sanyo. It is designed for precise temperature and humidity control applications in research and testing environments. The core function of the MIR-162 is to maintain stable environmental conditions for accurate and reliable experimental results.

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3 protocols using mir 162

1

Antioxidant Topical Formulation Development

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Paraffin oil (Merck KGOA, Darmstadt, Germany), acetyl alcohol (St. Louis, MO, USA), span 80 (Sigma-Aldrich, Darmstadt, Germany), 2,2-diphenyl-1-picrylhydrazyl (DPPH) (Sigma-Aldrich, Darmstadt, Germany), propyl paraben (Sigma-Aldrich, Darmstadt, Germany), Tween 80 (Sigma-Aldrich, Darmstadt, Germany), propylene glycol (Sigma-Aldrich, Darmstadt, Germany), methyl paraben (Sigma-Aldrich, Darmstadt, Germany), ethyl alcohol (Ethanol) (Sigma-Aldrich, Darmstadt, Germany), carbopol-940 (Sigma-Aldrich, St. Louis, MO, USA), triethanol amine (TEA) (Sigma-Aldrich, Darmstadt, Germany) were used in this research work. Purified water was manufactured at the laboratory of cosmeceuticals, Department of Pharmacy, IUB, Pakistan. Rotary evaporator (Heidolph, Co., Ltd., Schwabach, Germany, Bioevopeak Inc., Seattle, WA, USA), UV spectrophotometer (Boschaplatz 3 Presseck, 95355 Germany), microplate reader Synergy HT (BioTek Instrument, Stevens Creek Blvd, Santa Clara, CA, USA), refrigerator (Dawlance, Karachi, Pakistan), hot incubator (Sanyo MIR-162, Moriguchi City, Japan), digital humidity meter (TES Electronic Corp., Taipei, Taiwan), pH meter (WTW pH-197i, Weilheim, Germany), optical microscope (Eclipse E200, Nikon, Melville, NY, USA), and conductivity meter (WTW COND-197i, Weilheim, Germany) were used.
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2

Perioperative Body Temperature Monitoring

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Wireless thermometers connected to smartphones and iPads (iThermonitor; model WT705, Raiing Medical Company, Beijing, China) were used to monitor axillary temperature (monitoring every 4 seconds) throughout the surgery. iThermonitor was attached to the axilla with a hypoallergenic patch to avoid displacement. This device was approved by the FDA as a class II medical device.
The temperature of the operation room was maintained at 23 ± 1°C with relative humidity ranging from 40%-60%.20 (link) All patients arrived in the operating room covered with a single-layer cotton blanket and monitored body temperature. All operating tables are equipped with resistive heating mattresses. Before the induction of anesthesia, patients assigned to the aggressive warming group were covered with a single-layer cotton blanket and warmed using a resistive heating mattress (ASTOPAD OPT 100/104, Stihler Electronic GmbH, Germany) set to 37°C. In contrast, patients in the routine care group were only covered with a single-layer cotton blanket, and the resistive heating mattresses were not activated unless the core temperature decreased below 35.5°C. For both groups, all intravenous fluids were kept in an incubator (MIR-162; Sanyo, Osaka, Japan) heated to body temperature.
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3

Bacillus Culture Protocol for Research

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Bacillus culture. Bacillus cultures (Health Microbiology Laboratory, Research Center for Biology, Indonesian Institute of Sciences, Cibinong, Indonesia) were grown and rejuvenated in heterotrophic medium and incubated (Sanyo MIR-162, Osaka, Japan) at 37 °C for 24 h. The heterotrophic medium contained 3.0 g of agar, 3.6 g of tryptone, 1.0 g of NaCl, and 0.5 g of K 2 HPO 4 mixed in 200 mL of distilled water. The medium was sterilized in an autoclave (HVE 50 Hirayama, Japan) at 121 °C for 15 min. Cultures were incubated in the medium at 37 °C for ±48 h.
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