Fcγ block

Manufactured by Thermo Fisher Scientific

The Fcγ block is a laboratory equipment designed to facilitate protein binding studies. It provides a platform to investigate the interactions between the Fc region of antibodies and Fcγ receptors. The core function of the Fcγ block is to enable researchers to perform controlled and reproducible experiments in this area of immunology and protein research.

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Lab products found in correlation

F4 80 clone bm8, by Thermo Fisher Scientific (1 mentions) Ly6g clone 1a8, by BD (1 mentions) Cd11b clone m1 70, by Thermo Fisher Scientific (1 mentions) Cd11c clone n418, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by BD (1 mentions) Phycoerythrin conjugated anti cd11b, by BD (1 mentions) Allophycocyanin conjugated anti f4 80, by BioLegend (1 mentions) Fortessa flow cytometer, by BD (1 mentions)

2 protocols using fcγ block

Phagocytosis of Staphylococcus aureus by BMDCs

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PECs or blood leukocytes were blocked in Fcγ block (1 μg/ml; eBioscience) and then surface stained with fluorochrome-conjugated antibodies against Ly6G (clone 1A8; BD Bioscience), F4/80 (clone BM8; eBioscience), CD11c (clone N418; eBioscience), and CD11b (clone M1/70; eBioscience). Flow cytometric data were acquired with a BD FACSCanto II instrument (BD Biosciences) and analyzed using FlowJo software (Tree Star).
To assess the rate of S. aureus phagocytosis by BMDCs, cells that had been infected with CTV-labeled S. aureus for 30 min or 2 h were incubated with gentamicin (200 μg/ml) for 1 h, washed, and fixed in 2% PFA. The cells were then analyzed on BD FACSCanto II by gating on forward scatter and side scatter, and the percentage of CTV⁺ cells was assessed.

O'Keeffe K.M., Wilk M.M., Leech J.M., Murphy A.G., Laabei M., Monk I.R., Massey R.C., Lindsay J.A., Foster T.J., Geoghegan J.A, & McLoughlin R.M. (2015). Manipulation of Autophagy in Phagocytes Facilitates Staphylococcus aureus Bloodstream Infection. Infection and Immunity, 83(9), 3445-3457.

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Flow Cytometric Analysis of FAE-Induced Muscle Cells

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Total cells present in muscle after FAE injury were isolated by enzymatic digest as previously described.^{10 (link)} Cells were washed with 1% bovine serum albumin in phosphate-buffered saline (PBS); blocked with 2% bovine serum albumin in PBS with addition of Fcγ-block (eBioscience, San Diego, Calif); and stained with either a cocktail containing phycoerythrin-conjugated anti-CD11b (BD Biosciences, Franklin Lakes, NJ), peridinin-chlorophyll-conjugated anti-Gr1 (BioLegend, San Diego, Calif), fluorescein isothiocyanate-conjugated anti-Ly6C (BioLegend), and allophycocyanin-conjugated anti-F4/80 (BioLegend) or a cocktail of the corresponding isotype controls. Samples were run on the BD Fortessa Flow Cytometer (BD Biosciences) at The University of Texas at Austin Institute of Cell and Molecular Biology core facility with the forward scatter settings gated to exclude red blood cell-sized cells. Data were analyzed using FlowJo software (FlowJo, LLC, Ashland, Ore).

Hsieh P.L., Rybalko V., Baker A.B., Suggs L.J, & Farrar R.P. (2017). Recruitment and therapeutic application of macrophages in skeletal muscles after hind limb ischemia. Journal of vascular surgery, 67(6), 1908-1920.e1.

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