Lsm pascal microscope

Manufactured by Zeiss

Sourced in Germany

The LSM Pascal microscope is a high-performance confocal laser scanning microscope designed for advanced imaging applications. It offers precise and efficient optical sectioning capabilities, enabling researchers to capture detailed, three-dimensional images of biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Lsm image browser, by Zeiss (2 mentions) Bodipy c12, by Thermo Fisher Scientific (1 mentions) Lsm 710, by Zeiss (1 mentions) Lsm 880, by Zeiss (1 mentions) Anti β catenin, by BD (1 mentions) Anti flag m2, by Merck Group (1 mentions) Aqua poly mount, by Polysciences (1 mentions) Mitosox red, by Thermo Fisher Scientific (1 mentions) Cm h2dcfda, by Thermo Fisher Scientific (1 mentions) N sim super resolution microscope, by Nikon (1 mentions) Deltavision omx, by GE Healthcare (1 mentions) Vectashield, by Vector Laboratories (1 mentions)

Close spelling variants of this product

LSM 5 Pascal confocal microscope (85 citations) LSM 5 Pascal Laser Scanning Microscope (34 citations) LSM Pascal (31 citations) LSM 5 PASCAL laser scanning confocal microscope (17 citations) LSM 5 Pascal microscope (16 citations) LSM Pascal confocal microscope (9 citations) Zeiss LSM 5 Pascal Model Confocal Microscope (6 citations) LSM-5 Pascal 5 Axiovert 200 microscope (4 citations) LSM PASCAL confocal laser scanning microscope (4 citations) LSM-5 Pascal scanning confocal microscope (3 citations)

7 protocols using lsm pascal microscope

Cholesterol Staining in Fresh Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fresh unprocessed tissues were stained for CCs using cholesteryl Bodipy-C12 fluorescent dye [15 (link)]. Fluorescence images were acquired using a Zeiss Pascal LSM microscope (Carl Zeiss, Inc., Jena, Germany).

Abela G.S., Katkoori V.R., Pathak D.R., Bumpers H.L., Leja M., ul Abideen Z., Boumegouas M., Perry D., Al-Janadi A., Richard J.E., Barnaba C, & Medina Meza I.G. (2023). Cholesterol crystals induce mechanical trauma, inflammation, and neo-vascularization in solid cancers as in atherosclerosis. American Heart Hournal Plus: Cardiology Research and Practice, 35, 100317.

+ Open protocol

+ Expand

Visualizing Cholesterol Localization in S. aureus

Check if the same lab product or an alternative is used in the 5 most similar protocols

S. aureus bacteria were incubated with and without CCs for 3 h at 37°C in broth. After incubation, samples were fixed with 4% glutaraldehyde and then stained for cholesterol using a green, fluorescent dye (cholesteryl BODIPY-C12, Invitrogen, Eugene, OR) at a 1/100 dilution (75% ethanol) in a test tube for 3 minutes [7 (link)]. Samples were then transferred to a slide incubator chamber and visualized. Confocal fluorescence images of the bacteria were acquired using a Zeiss Pascal LSM microscope (Carl Zeiss, Inc, Jena, Germany). The green fluorescence was excited with the 488 nm argon laser line, and emission was collected using a 505 to 530 nm band-pass filter.

Boumegouas M., Raju M., Gardiner J., Hammer N., Saleh Y., Al-Abcha A., Kalra A, & Abela G.S. (2022). Interaction between bacteria and cholesterol crystals: Implications for endocarditis and atherosclerosis. PLoS ONE, 17(2), e0263847.

+ Open protocol

+ Expand

Immunofluorescence Staining of β-Catenin and M2-Flag

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells grown on coverslips were collected for immunofluorescence 24 h after transfection. Cells were washed in PBS and then fixed in 4% formaldehyde for 5 min. Cells were then permeabilized with 0.1% TritonX-100 in PBS for 5 min. After 30 min in block buffer (0.01% NGS in PBS), cells were incubated in primary antibody for 1–2 h, washed with PBS, and then incubated in secondary antibody for 1–2 h. Cells were mounted on microscope slides in Aqua polymount (Polyscience). Primary antibodies used: anti-βCatenin (BD Transduction, 1:800) and anti-M2-Flag (Sigma, 1:1000). Immunostained cells were imaged on an LSM Pascal microscope (Zeiss), an LSM 710 (Zeiss), or an LSM 880 (Zeiss). All images were processed using Fiji to create maximum intensity projections, and Photoshop CS6 (Adobe, San Jose, CA) was used to adjust input levels so that the signal spanned the entire output grayscale and to adjust brightness and contrast.

Schaefer K.N., Pronobis M.I., Williams C.E., Zhang S., Bauer L., Goldfarb D., Yan F., Major M.B, & Peifer M. (2020). Wnt regulation: exploring Axin-Disheveled interactions and defining mechanisms by which the SCF E3 ubiquitin ligase is recruited to the destruction complex. Molecular Biology of the Cell, 31(10), 992-1014.

+ Open protocol

+ Expand

Mitochondrial Reactive Oxygen Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were incubated with 5 μM MitoSOX Red (Molecular Probes, Eugene, OR, USA) for 20 min at 37 °C, with 1 μM CM-H₂DCFDA (Molecular Probes, Eugene, OR, USA) for 5 min or 100 nM TMRM (Molecular Probes, Eugene, OR, USA) for 30 min and were subsequently washed with an extracellular solution (Tyrode) that contained 1.2 mM Ca²⁺ to remove the excess of dye. The images were recorded with confocal line-scan imaging using a Zeiss LSM Pascal microscope, with a 40X water-immersive objective. Two-dimensional imaging mode was used, with excitation at 488 and emission at > 517 nm, for CM-H₂DCFDA, alternating with excitation at 532 nm, and emission at > 560 nm for TMRM. Time-lapse x,y images were acquired at 1024 bit resolution and at the sampling rate of 507 ms per frame.

Pereira C.L., Ximenes C.F., Merlo E., Sciortino A.S., Monteiro J.S., Moreira A., Jacobsen B.B., Graceli J.B., Ginsburg K.S., Ribeiro RF J.u.n.i.o.r., Bers D.M, & Stefanon I. (2019). Cardiotoxicity of environmental contaminant tributyltin involves myocyte oxidative stress and abnormal Ca2+ handling. Environmental pollution (Barking, Essex : 1987), 247, 371-382.

+ Open protocol

+ Expand

Imaging Techniques for Fluorescence Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunostained samples were imaged on a LSM Pascal microscope (Zeiss) and processed with the LSM image browser (Zeiss). SIM microscopy was carried out on the N-SIM superresolution microscope (Nikon) using 4% formaldehyde-fixed samples mounted in Aquapolymount. Images were processed using Imaris 5.5 (Bitplane), ImageJ, and the LSM image browser.

Pronobis M.I., Deuitch N., Posham V., Mimori-Kiyosue Y, & Peifer M. (2017). Reconstituting regulation of the canonical Wnt pathway by engineering a minimal β-catenin destruction machine. Molecular Biology of the Cell, 28(1), 41-53.

+ Open protocol

+ Expand

Quantifying Zebrafish Brain Volumes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Images of zebrafish (6 dpf) expressing the photoconvertible protein Kaede pan-neuronally driven by the ELAV3 promoter (Sato et al., 2006 (link)) were used to determine the whole-brain volume and volumes of brain regions in zebrafish exposed to BPA. To image zebrafish brains, an LSM Pascal microscope (Zeiss) was used (488-nm excitation) to construct a whole-brain image stack using z-stacks (2-μm slices) and tiling. The tiles were stitched together after the images were taken. In addition, we used depth-dependent adaptive illumination to ensure adequate detection of deeper brain regions. The calculated volumes from the 2-μm slices were then summed to determine the total volume for each region (forebrain, midbrain, and hindbrain), and these values were then added to quantify the total brain volume.

Scaramella C., Alzagatiti J.B., Creighton C., Mankatala S., Licea F., Winter G.M., Emtage J., Wisnieski J.R., Salazar L., Hussain A., Lee F.M., Mammootty A., Mammootty N., Aldujaili A., Runnberg K.A., Hernandez D., Zimmerman-Thompson T., Makwana R., Rouvere J., Tahmasebi Z., Zavradyan G., Campbell C.S., Komaranchath M., Carmona J., Trevitt J., Glanzman D, & Roberts A.C. (2022). Bisphenol A Exposure Induces Sensory Processing Deficits in Larval Zebrafish during Neurodevelopment. eNeuro, 9(3), ENEURO.0020-22.2022.

+ Open protocol

+ Expand

Microscopic Imaging and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunostained samples were imaged on a LSM Pascal microscope (Zeiss) and processed with the LSM image browser (Zeiss, Germany). SIM microscopy was carried out on the Deltavision OMX (GE Healthcare Life Sciences, Pittsburgh PA) using 4% formaldehyde fixed samples mounted in Vectashield (Vector, Burlingame CA) following manufacturer's protocol. Images were processed using Imaris 5.5, ImageJ and the LSM image browser. PhotoshopCS4 (Adobe, San Jose, CA) was used to adjust levels so that the range of signals spanned the entire output grayscale and to adjust brightness and contrast.

Pronobis M.I., Rusan N.M, & Peifer M. (2015). A novel GSK3-regulated APC:Axin interaction regulates Wnt signaling by driving a catalytic cycle of efficient βcatenin destruction. eLife, 4, e08022.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!