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Alkaline phosphatase

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Alkaline phosphatase is an enzyme that catalyzes the hydrolysis of phosphate esters in an alkaline environment. It is commonly used in diagnostic assays to measure its activity levels in various biological samples.

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Lab products found in correlation

Creatinine, by Biodiagnostic (3 mentions) Aspartate aminotransferase, by Biodiagnostic (2 mentions) Albumin, by Biodiagnostic (2 mentions) Ascorbic acid, by Honeywell (1 mentions) Ursolic acid, by Merck Group (1 mentions) Butylated hydroxytoluene, by Merck Group (1 mentions) 2 4 dinitrophenylhydrazine, by Merck Group (1 mentions) Glutathione (gsh), by Merck Group (1 mentions) Biozol reagent, by Thermo Fisher Scientific (1 mentions) Syber green 1 step qrt pcr kit, by Thermo Fisher Scientific (1 mentions) Folin ciocalteau reagent, by Merck Group (1 mentions) Gallic acid, by Merck Group (1 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions) Rutin, by Merck Group (1 mentions) 5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions) 2 2 azino bis, by Merck Group (1 mentions) Total protein, by Biodiagnostic (1 mentions) Sensifast cdna synthesis kit, by Meridian Bioscience (1 mentions) Alanine aminotransferase, by Biodiagnostic (1 mentions) Urea, by Biodiagnostic (1 mentions)

4 protocols using alkaline phosphatase

1

Antioxidant and Anti-inflammatory Assays

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Rutin (RU), gallic acid (GA), catechin, ursolic acid (UA), Folin–Ciocalteau reagent, 2,2 diphenyl-1-picrylhydrazyl (DPPH), 2,4 dinitrophenyl hydrazine (DNPH), 5, 5′, dithiobis-2-nitrobenzoic acid (DTNB), butylated hydroxytoluene (BHT), CCl4 (reagent grade, 99.9%), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), NADPH, and GSH were acquired from Sigma-Aldrich, St Louis, MO, USA. Thiobarbituric acid (TBA) was gained from El-Nasr Pharmaceutical Chemicals Co. (Alex., Egypt). Ascorbic acid [(Asc): vitamin C] and Trolox were bought from Riedel-de Haën, Germany. Biozol reagent was purchased from Invitrogen, CA, USA. SYBER Green 1-step qRT-PCR Kit was purchased from Thermo Scientific, USA. Primers for tumor necrosis factor (TNF)-α, nuclear factor-kappa B (NF-κB), transforming growth factor (TGF)-β1, interleukin (IL)-6, and the tumor suppressor gene p53 were acquired from Bioneer, Korea. Kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), albumin, creatinine, urea, triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c) were gained from Biodiagnostic, Cairo, Egypt.
Shaban N.Z., Awad O.M., Fouad G.M., Hafez A.M., Abdul-Aziz A.A, & El-Kot S.M. (2022). Prophylactic and curative effects of Carica papaya Linn. pulp extract against carbon tetrachloride-induced hepatotoxicity in male rats. Environmental Science and Pollution Research International, 30(10), 27815-27832.
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2

Hepatoprotective and Antioxidant Evaluation

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Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein, albumin, creatinine, urea, and uric acids were purchased from Biodiagnostics Company (Dokki, Giza, Egypt). The RNA extraction Kit was purchased from Introbio Company, Cat.No. 17063. Reverse transcriptase Polymerase chain reaction (RT-PCR) sensiFAST™ cDNA synthesis kit (Bioline, United Kingdom). All used primers are commercially based. Aflatoxin from the Mycology and Mycotoxins Department, Animal Health Research Institute (ARC), Dokki, Egypt. Curcumin Nanoparticles (NT-Cur-NPs) were purchased from Nanotech Egypt for photo- electronics at the city of 6 October, Al Giza. Saccharomyces cerevisiae cell wall extract (100%, Beta-glucan: 20% minimum &Manan Oligosaccharides: 20% minimum.) was obtained from IBEX Group for animal nutrition and health products and produced by Shandong Bio Sunkeen Co., Ltd (a state-owned high-tech enterprise that integrates R&D, production and sales with rich experience of yeast and nature yeast products.
Ashry A., Taha N.M., Lebda M.A., Abdo W., El-Diasty E.M., Fadl S.E, & Morsi Elkamshishi M. (2022). Ameliorative effect of nanocurcumin and Saccharomyces cell wall alone and in combination against aflatoxicosis in broilers. BMC Veterinary Research, 18, 178.
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3

Cypermethrin Biochemical Evaluation Protocol

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Cypermethrin (95%) was obtained from Jiangsu Yangnong Chemical Co., Ltd., China. The assay kits used for biochemical measurements of total protein, albumin, urea nitrogen, aspartate aminotransferases (EC 2.6.1.1.), alanine aminotransferases (EC 2.6.1.2), alkaline phosphatase (EC 3.1.3.1), creatinine and gamma-glutamyl transferase (EC 2.3.2.2) in serum were purchased from Bio-Diagnostic Co., Egypt. Kit of total protein was obtained from Stanbio Laboratory, Texas, USA. All other chemicals were of reagent grades and obtained from Sigma-Aldrich and local scientific distributors in Egypt.
Mossa A.T., Ibrahim F.M., Mohafrash S.M., Abou Baker D.H, & El Gengaihi S. (2015). Protective Effect of Ethanolic Extract of Grape Pomace against the Adverse Effects of Cypermethrin on Weanling Female Rats. Evidence-based Complementary and Alternative Medicine : eCAM, 2015, 381919.
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4

Biochemical Profiling of Serum Markers

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Serum biochemical concentrations were measured using commercial test kits in accordance with standard protocols. The serum amyliod A (SAA) and plasma fibrinogen (Fb) concentrations were assessed using IBL International Crop (Canada)® ELISA kits, and haptoglobin (Hp) by Eagle Biosciences (Columbia) ELISA kits. The total protein, albumin, glucose, cholesterol, urea, and creatinine were determined by kits from Gamma Trade Company, Egypt. The insulin levels were measured by kits from NeoBiolab, Cambridge, USA. For T3 and T4 commercial test, Malondialdehyde (MDA), catalase (CAT), reduced glutathione (GSH), super oxide dismutase (SOD), Glutathione peroxidase (GPx) and alkaline phosphatase (ALP) were provided by Bio diagnostic, Egypt. Betahydroxybutyrate (BHBA) provided by Cayman chemical Company, USA. NEFA kits from FUJIFILM Wako Chemicals, USA. The IGF1 kits from Wuhan Fine Biotech. Co, China. Total bilirubin from Atlas Medical, Germany. Globulin was calculated by subtracting albumin values from total serum protein.
El-Sayed A.A., Sallam A.M, & Abou-Soliman I. (2024). Metabolic profile and gene expression pattern of cytokines and antioxidants markers during different physiological stages in Barki ewes. BMC Veterinary Research, 20, 206.
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