Human cord blood mononuclear cells were isolated by Ficoll-Paque (Axis-Shield, Oslo, Norway) density gradient centrifugation from blood of health volunteer donors. CD4+ T cells were purified by using relevant magnetic MicroBead kits (Miltenyi Biotec GmbH, Bergisch Gladbach, German) according to the manufacturer's instructions. The purity of CD4+ T cells was more than 95%.
Ficoll paque
Manufactured by Axis-Shield
Sourced in Norway
Ficoll-Paque is a density gradient medium used for the separation and isolation of mononuclear cells from whole blood or bone marrow. It is a sterile, pyrogen-tested, and endotoxin-tested solution of Ficoll and sodium diatrizoate.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ficoll paque
1
Isolation of CD4+ T Cells from Cord Blood
2
CD4+ T Cell Isolation from ITP Patients
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Human mononuclear cells from patients with ITP were isolated by Ficoll-Paque (Axis-Shield). Then, CD4+T cells were obtained with magnetic MicroBead kits (Miltenyi Biotec). The purity of CD4+T cells was more than 95% (data not shown).
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