Inhibitors including HIF‐1α and tumor necrosis factor alpha (TNF‐α) were obtained (VH‐298, MedChemExpress; HY‐100947, Methylthiouracil; HY‐B0513, MedChemExpress). OS‐9 overexpression or control SMMC‐7721 were treated with the two inhibitors at 20 μM or 20 μM, respectively, and then invasion and migration assay were administrated.
Vh 298
Manufactured by MedChemExpress
VH-298 is a laboratory centrifuge designed for separating and concentrating samples. It can be used to pellet cells, organelles, and macromolecules from complex solutions. The centrifuge has a maximum speed of 14,000 rpm and can achieve up to 20,000 x g of centrifugal force.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using vh 298
1
Inhibiting HIF-1α and TNF-α in OS-9 Overexpressing Cells
2
Affinity Reagent Procurement for Biochemical Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The Tb-anti-GST antibody, 1,4-dithiothreitol
(DTT, 1 M), Tris (1 M, pH 7.5), and DMSO were purchased from Fisher
Scientific (Pittsburgh, PA). HEPES (1 M, pH 7.4) was purchased from
Teknova, Inc. (Hollister, CA). Triton X-100, Tween-20, and bovine
serum albumin were purchased from Sigma-Aldrich (St. Louis, MO). VH-298,
VH032-amine, Me-VH032-amine, and VH032-PEG4-NH2 were purchased
from MedChemExpress, LLC (Monmouth Junction, NJ). BOC-VH032 was purchased
from LabNetwork (Cambridge, MA). VH032 phenol and thalidomide-4′-O-acetamido-alkylC4-amine were purchased from Bio-Techne
Corporation (Minneapolis, MN). MZ1, dBET1, and (+)-JQ1 were purchased
from Cayman Chemical (Ann Arbor, MI). Echo 384-well low dead volume
compound plates were purchased from Labcyte, Inc. (San Jose, CA).
384-well, black low-volume assay plates were purchased from Corning
Incorporated Life Sciences (Tewksbury, MA).
(DTT, 1 M), Tris (1 M, pH 7.5), and DMSO were purchased from Fisher
Scientific (Pittsburgh, PA). HEPES (1 M, pH 7.4) was purchased from
Teknova, Inc. (Hollister, CA). Triton X-100, Tween-20, and bovine
serum albumin were purchased from Sigma-Aldrich (St. Louis, MO). VH-298,
VH032-amine, Me-VH032-amine, and VH032-PEG4-NH2 were purchased
from MedChemExpress, LLC (Monmouth Junction, NJ). BOC-VH032 was purchased
from LabNetwork (Cambridge, MA). VH032 phenol and thalidomide-4′-O-acetamido-alkylC4-amine were purchased from Bio-Techne
Corporation (Minneapolis, MN). MZ1, dBET1, and (+)-JQ1 were purchased
from Cayman Chemical (Ann Arbor, MI). Echo 384-well low dead volume
compound plates were purchased from Labcyte, Inc. (San Jose, CA).
384-well, black low-volume assay plates were purchased from Corning
Incorporated Life Sciences (Tewksbury, MA).
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