Ammonium sulfide

After 24 hours of incubation in 4% PFA, the retinas were washed in tris maleate buffer (pH 7.2) on ice prior to incubation in ADPase incubation medium containing 3.0 mM lead nitrate and 6.0 mM magnesium chloride (Sigma Chemical Co., St. Louis, MO) in tris maleate buffer (pH 7.2). After incubation, the retinas were washed with tris maleate buffer prior to addition of diluted ammonium sulfide (Fisher Scientific, Silver Spring, MD, USA) for 1 min. The retinas were washed again in tris maleate buffer and flat-mounted on a microscope slide with PBS and glycerin. All images were captured using an Olympus BX53 microscope, DP72 digital camera, and CellSens Dimension imaging software (version 2.1) from Olympus America, Inc. (Center Valley, PA, USA), attached to a HP Z44 computer.

After 24 h of incubation in 4% PFA, the retinas were washed in tris-maleate buffer (pH 7.2) on ice prior to incubation in an ADPase incubation medium containing 3.0 nM of lead nitrate and 6.0 mM of magnesium chloride (Sigma Chemical Co., St. Louis, MO, USA) in tris-maleate buffer (pH 7.2). After incubation, the retinas were washed with tris-maleate buffer prior to the addition of diluted ammonium sulfide (Fisher Scientific, Silver Spring, MD, USA) for 1 min. The retinas were washed again in tris-maleate buffer and flatmounted on a microscope slide with PBS and glycerin. All of the images were captured using an Olympus BX53 microscope, DP72 digital camera, and CellSens Dimension imaging software (version 2.1) from Olympus America, Inc. (Center Valley, PA, USA), attached to a HP Z44 computer.