Log In Sign up
The largest database of trusted experimental protocols

Anti pu 1 antibody d 19

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in United States

The Anti-PU.1 antibody (D-19) is a laboratory tool used for detecting and analyzing the PU.1 protein, a transcription factor involved in the regulation of gene expression. The antibody is designed to specifically bind to and identify the PU.1 protein in various experimental techniques such as Western blotting, immunoprecipitation, and immunohistochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Anti irf8 antibody c19, by Santa Cruz Biotechnology (1 mentions) Anti β actin antibody ac 15, by Merck Group (1 mentions)

Spelling variants of this product

Anti-PU.1 (12 citations) Anti-PU.1 antibody (5 citations) PU.1 antibody (2 citations)

2 protocols using anti pu 1 antibody d 19

1

Western Blot Analysis of PU.1, IRF4, and IRF8

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting analysis was performed as previously described20 (link). Following antibodies were used: anti-PU.1 antibody (D-19), anti-IRF4 antibody (M-17), and anti-IRF8 antibody (C-19), (Santa Cruz Biotechnology, Santa Cruz, CA), and anti-β-actin antibody (AC-15, Sigma-Aldrich).
Yashiro T., Nakano S., Nomura K., Uchida Y., Kasakura K, & Nishiyama C. (2019). A transcription factor PU.1 is critical for Ccl22 gene expression in dendritic cells and macrophages. Scientific Reports, 9, 1161.
+ Open protocol
+ Expand
2

ChIP Assay for PU.1 Transcription Factor

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP assay was performed according to a previously described protocol [17]. Anti‐PU.1 antibody (D19; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and goat IgG (Invitrogen, Carlsbad, CA, USA) were used. Quantitative PCR of chromosomal DNA was performed as described in subsection Quantitative RT‐PCR. The sequences of primer sets used were previously described [16].
Yashiro T., Takeuchi H., Kasakura K, & Nishiyama C. (2020). PU.1 regulates Ccr7 gene expression by binding to its promoter in naïve CD4+ T cells. FEBS Open Bio, 10(6), 1115-1121.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!