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Stereotaxic frame at 45

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The Stereotaxic frame at 45° is a laboratory instrument used for precise positioning and immobilization of small animals, such as rodents, during surgical or experimental procedures. The frame is designed to hold the animal's head at a 45-degree angle, allowing for targeted access to specific brain regions. The core function of this device is to provide a stable, reproducible, and standardized platform for conducting research and procedures that require precise positioning and orientation of the subject.

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Stereotaxic frame (536 citations)

2 protocols using stereotaxic frame at 45

1

Near-Infrared Imaging of Traumatic Brain Injury

Cited 1 time
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The TBI was performed based on the previously reported procedures with modifications43 (link). Briefly, the benchmark stereotaxic impactor was mounted on a stereotaxic frame at 45° (David Kopf Instruments, Tujunga, CA, USA). Anesthetized mice were placed on a customized foam mold in prone position. To induce TBI, the tip was driven towards mouse head at a speed of 4.0–4.5 mm/s, a dwell time of 0.2 s set by the electronic control box and an impact depth of 3 mm adjusted by the stereotaxic device. After 2 h recovery, 4–6-week-old C57BL/6 was intravenously injected with 200 μL anti-CD11b-PEGylated PbS/CdS CSQD (O.D. = 0.5 at 808 nm) and monitored by wide-field system and oblique NIR-II LSM mounted with a 5X illumination objective and a 10X imaging objective 24 h post injection. The Z scanning increment, exposure time and excitation and emission wavelengths were summarized in Supplementary Table 2.
Wang F., Wan H., Ma Z., Zhong Y., Sun Q., Tian Y., Qu L., Du H., Zhang M., Li L., Ma H., Luo J., Liang Y., Li W.J., Hong G., Liu L, & Dai H. (2019). Light Sheet Microscopy in the Near-Infrared II Window. Nature methods, 16(6), 545-552.
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2

Near-Infrared Imaging of Traumatic Brain Injury

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The TBI was performed based on the previously reported procedures with modifications43 (link). Briefly, the benchmark stereotaxic impactor was mounted on a stereotaxic frame at 45° (David Kopf Instruments, Tujunga, CA, USA). Anesthetized mice were placed on a customized foam mold in prone position. To induce TBI, the tip was driven towards mouse head at a speed of 4.0–4.5 mm/s, a dwell time of 0.2 s set by the electronic control box and an impact depth of 3 mm adjusted by the stereotaxic device. After 2 h recovery, 4–6-week-old C57BL/6 was intravenously injected with 200 μL anti-CD11b-PEGylated PbS/CdS CSQD (O.D. = 0.5 at 808 nm) and monitored by wide-field system and oblique NIR-II LSM mounted with a 5X illumination objective and a 10X imaging objective 24 h post injection. The Z scanning increment, exposure time and excitation and emission wavelengths were summarized in Supplementary Table 2.
Wang F., Wan H., Ma Z., Zhong Y., Sun Q., Tian Y., Qu L., Du H., Zhang M., Li L., Ma H., Luo J., Liang Y., Li W.J., Hong G., Liu L, & Dai H. (2019). Light Sheet Microscopy in the Near-Infrared II Window. Nature methods, 16(6), 545-552.
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