Recombinant murine chemerin

Tanon 5200 multi automatic chemiluminescence/fluorescence image analysis system was purchased from Tanon Science and Technology Co., Ltd. The fluorescence inverted microscope was obtained from Olympus Corporation. High-glucose (95%) DMEM was acquired from Beijing Solarbio Science & Technology Co., Ltd. Recombinant murine chemerin was purchased from R&D Systems, Inc. Penicillin/streptomycin and PBS solutions were acquired from Beijing Solarbio Science & Technology Co., Ltd. High performance RIPA lysis buffer solution and western and immunoprecipitation cell lysis solution were purchased from Beyotime Institute of Biotechnology. BCA protein quantification and ECL kits were obtained from MultiSciences (Lianke) Biotech Co., Ltd. The PVDF membrane (0.45 µm) was obtained from MilliporeSigma. Rat MMP-3 (cat. no. SEKR-0067-96T), MMP-13 (cat. no. SEKR-0035-96T), TNF-α (cat. no. SEKR-0009-96T), IL-1β (cat. no. SEKR-0002-96T) and IL-6 (cat. no. SEKR-0005-96T) ELISA kits were acquired from Beijing Solarbio Science & Technology Co., Ltd.

APC-conjugated anti-CD11c, anti-CD44, and anti–IFN-γ mAbs; APC-Cy7–conjugated anti-B220, anti-CD4, and anti-CD11c mAbs; FITC-conjugated anti–MHC class II mAb; PB-conjugated anti-CD11c, anti-CD45, and anti–MHC class II mAbs; PE-conjugated anti-CD8, anti-CD62L, and anti-TCRβ mAbs; PE-Cy7–conjugated anti-CD45 and anti–MHC class II mAbs; and annexin V and PI were purchased from eBioscience. BLT1 antagonist U-75302 was purchased from Cayman Chemical. A ChemR23 agonist (recombinant murine chemerin) was purchased from R&D Systems. BLT1 antagonist ONO-4057 was provided by Ono Pharmaceutical Co., Ltd. RvE1 was synthesized as previously described (Ogawa and Kobayashi, 2009 (link)).