Serum clot activator vacuette tubes

For serum chemistries, whole blood was collected into Serum Clot Activator Greiner Vacuette tubes (Greiner Bio-One, Monroe, NC, USA). Tubes were allowed to clot for at least 10 min and the serum separated in a centrifuge set at 1800× g for 10 min at ambient temperature. The required volume of serum was removed for chemistry analysis using a General Chemistry 13 panel (Abaxis, Union City, CA, USA) on a Piccolo Point-Of-Care Analyzer (Abaxis). Serum was removed from the clot within 1 h of centrifugation and was analyzed within 12 h of collection.
For hematology, whole blood was collected into Greiner Vacuette blood tubes containing K3 EDTA as an anti-coagulant. Hematology was performed on a VETSCAN^® HM5 hematology analyzer (Abaxis) within 4 h of collection.

For serum chemistries, whole blood was collected into Serum Clot Activator Greiner Vacuette tubes (Greiner Bio-One, Monroe, NC). Tubes were allowed to clot for at least 10 min and the serum separated in a centrifuge set at 1800 × g for 10 min at ambient temperature. The required volume of serum was removed for chemistry analysis using a General Chemistry 13 panel (Abaxis, Union City, CA) on a Piccolo Point-Of-Care Analyzer (Abaxis, Union City, CA). Serum was removed from the clot within 1 hour of centrifugation and was analyzed within 12 hours of collection.
For hematology, whole blood was collected into Greiner Vacuette blood tubes containing K3 EDTA as an anti-coagulant. Hematology was performed on VETSCAN® HM5 hematology analyzer (Abaxis, Union City, CA) within 4 hours of collection. In addition, 100 uL of whole blood was added to 300 uL of TRIzol® LS (Thermo Fisher Scientific, Waltham, MA) for RNA isolation for qRT-PCR.