The anti CD45 (BD Pharmingen, # 550539) was diluted 1:25, isotype control antibodies (rat IgG2b 1:400; Biolegend, # 400622) served as a negative control. Deparaffinized sections were heated in a microwave oven (800W) in DAKO retrieval solution (Target Retrieval Solution DAKO by BIOZOL, Germany # S1699). Slides were incubated 1 h at room temperature with the primary antibody, rinsed, and then incubated for 30 min at room temperature with the respective secondary rabbit α rat-biotinylated antibody (Jackson Immuno Research # 312-065-048). CD45 antibody binding sites were detected by incubation of the sections with the streptavidin-alkaline phosphate complex (ABC-AP # AK-5000; Vector Laboratories, Burlingame, CA, USA) for 30 min. For visualizing enzyme reactivity of the alkaline phosphate complex, liquid permanent red (DAKO by BIOZOL, Germany # K0640) was applied to the sections for 15 min under visual control. Then they were washed again under running tap water and finally rinsed in distilled water. After counterstaining with hemalaun, they were finally mounted using a resinous permanent mounting medium (Eukitt, Kindler GmbH, Freiburg, Germany).
Rabbit α rat biotinylated antibody
Manufactured by Jackson ImmunoResearch
Rabbit α rat-biotinylated antibody is a secondary antibody produced in rabbits and conjugated with biotin. It is designed to specifically bind to rat primary antibodies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit α rat biotinylated antibody
1
Immunohistochemical Detection of CD45
2
Immunohistochemical Staining of CD45 Leukocyte Marker
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Pan-leukocyte marker CD45
The anti CD45 (BD Pharmingen, # 550539) was diluted 1:25, isotype control antibodies (rat IgG2b 1:400; Biolegend, # 400622) served as a negative control. Depara nised sections were heated in a microwave oven (800W) in DAKO retrieval solution (Target Retrieval Solution DAKO by BIOZOL, Germany # S1699). Slides were incubated 1 h at room temperature with the primary antibody, rinsed and then incubated for 30 min at room temperature with the respective secondary rabbit α rat-biotinylated antibody (Jackson Immuno Research # 312-065-048). CD45 antibody binding sites were detected by incubation of the sections with the streptavidin-alkaline phosphate complex (ABC-AP # AK-5000; Vector Laboratories, Burlingame, CA, USA) for 30 min. For visualizing enzyme reactivity of the alkaline phosphate complex, liquid permanent red (DAKO by BIOZOL, Germany # K0640) was applied to the sections for 15 min under visual control. Then they were washed again under running tap water and nally rinsed in distilled water.
After counterstaining with hemalaun, they were nally mounted using a resinous permanent mounting medium (Eukitt, Kindler GmbH, Freiburg, Germany).
The anti CD45 (BD Pharmingen, # 550539) was diluted 1:25, isotype control antibodies (rat IgG2b 1:400; Biolegend, # 400622) served as a negative control. Depara nised sections were heated in a microwave oven (800W) in DAKO retrieval solution (Target Retrieval Solution DAKO by BIOZOL, Germany # S1699). Slides were incubated 1 h at room temperature with the primary antibody, rinsed and then incubated for 30 min at room temperature with the respective secondary rabbit α rat-biotinylated antibody (Jackson Immuno Research # 312-065-048). CD45 antibody binding sites were detected by incubation of the sections with the streptavidin-alkaline phosphate complex (ABC-AP # AK-5000; Vector Laboratories, Burlingame, CA, USA) for 30 min. For visualizing enzyme reactivity of the alkaline phosphate complex, liquid permanent red (DAKO by BIOZOL, Germany # K0640) was applied to the sections for 15 min under visual control. Then they were washed again under running tap water and nally rinsed in distilled water.
After counterstaining with hemalaun, they were nally mounted using a resinous permanent mounting medium (Eukitt, Kindler GmbH, Freiburg, Germany).
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