Discovery ultra processor

Manufactured by Roche

The Discovery Ultra processor is a laboratory equipment designed for high-throughput DNA sequencing. It provides automated sample preparation and data analysis capabilities to support genomic research and next-generation sequencing applications.

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Lab products found in correlation

Discovery chromomap dab detection kit, by Roche (2 mentions) Omnimap anti rabbit hrp, by Roche (2 mentions) Autostainer xl, by Leica (1 mentions) Pannoramic 250 flash, by 3DHISTECH (1 mentions) Caseviewer software, by 3DHISTECH (1 mentions) Discovery xt processor, by Roche (1 mentions) Pannoramic viewer, by 3DHISTECH (1 mentions)

Spelling variants of this product

Discovery Ultra (119 citations) Discovery Ultra automated processor (5 citations)

3 protocols using discovery ultra processor

Testis Tissue Processing and Staining Protocol

Cited 2 times

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Fixation and testis tissue processing were performed as described (Jain et al. 2018 (link)). Periodic acid Schiff (PAS) staining, immunohistochemical TUNEL assay, and immunofluorescent staining were performed by the MSK Molecular Cytology Core Facility. PAS staining was done using the Autostainer XL (Leica Microsystems) automated stainer for PAS with hematoxylin counterstain. TUNEL assay was done using the Discovery XT processor (Ventana Medical Systems) as described previously (Jain et al. 2017 (link)). Immunofluorescent staining was done with the Discovery Ultra processor (Ventana Medical Systems) and is detailed in the Supplemental Material. Stained slides were digitized using Pannoramic Flash 250 (3DHistech) with a 40× objective. Images were produced and analyzed using Pannoramic Viewer or CaseViewer software (3DHistech). Histological examination of somatic tissues is described in the Supplemental Material.

Saito Y., Hawley B.R., Puno M.R., Sarathy S.N., Lima C.D., Jaffrey S.R., Darnell R.B., Keeney S, & Jain D. (2022). YTHDC2 control of gametogenesis requires helicase activity but not m6A binding. Genes & Development, 36(3-4), 180-194.

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IHC Analysis of Ki-67 and Cleaved Caspase-3

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Immunohistochemistry (IHC) for Ki-67 and cleaved caspase 3 was performed in Molecular Cytology Core Facility of Memorial Sloan Kettering Cancer Center using a Discovery Ultra processor (Ventana Medical Systems). The Ki-67 probe (rabbit monoclonal Ki-67 antibody, Cell Signaling, #9027) was used at 1 ug/ml concentration and the cleaved caspase 3 probe (rabbit polyclonal cleaved caspase-3 antibody, Cell Signaling, #9661) was used at 0.2ug/ml concentration. Incubation with the primary antibody was done for 4 hours, followed by 60 minutes incubation with an OmniMap anti-rabbit HRP (Roche Diagnostics Cat#760–4311) secondary antibody. Discovery ChromoMap DAB detection kit (Roche Diagnostics) was used according to the manufacturer instructions. LK-2 xenograft tumors treated with vehicle, everolimus, and TAK-228 were tested. Tumors were harvested at the end of treatment (~ day 23). Slides were reviewed and scored by two pathologists, and differences assessed for statistical significance using GraphPad Prism 9 (unpaired t-tests).

Paik P.K., Fan P.D., Qeriqi B., Namakydoust A., Daly B., Ahn L., Kim R., Plodkowski A., Ni A., Chang J., Fanaroff R., Ladanyi M., de Stanchina E, & Rudin C.M. (2022). Targeting NFE2L2/KEAP1 mutations in advanced non-small cell lung cancer with the TORC1/2 inhibitor TAK-228. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, 18(4), 516-526.

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Quantification of Proliferation and Apoptosis in Xenograft Tumors

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Immunohistochemistry (IHC) for Ki-67 and cleaved caspase 3 was performed in Molecular Cytology Core Facility of Memorial Sloan Kettering Cancer Center using a Discovery Ultra processor (Ventana Medical Systems). The Ki-67 probe (rabbit monoclonal Ki-67 antibody, Cell Signaling, #9027) was used at 1 ug/ml concentration and the cleaved caspase 3 probe (rabbit polyclonal cleaved caspase-3 antibody, Cell Signaling, #9661) was used at 0.2ug/ml concentration. Incubation with the primary antibody was done for 4 hours, followed by 60 minutes incubation with an OmniMap anti-rabbit HRP (Roche Diagnostics Cat#760-4311) secondary antibody. Discovery ChromoMap DAB detection kit (Roche Diagnostics) was used according to the manufacturer instructions. LK-2 xenograft tumors treated with vehicle, everolimus, and TAK-228 were tested. Tumors were harvested at the end of treatment (~ day 23). Slides were reviewed and scored by two pathologists, and differences assessed for statistical significance using GraphPad Prism 9 (unpaired t-tests).

Paik P.K., Fan P.D., Qeriqi B., Namakydoust A., Daly B., Ahn L., Kim R., Plodkowski A., Ni A., Chang J., Fanaroff R., Ladanyi M., de Stanchina E, & Rudin C.M. (2023). Targeting NFE2L2/KEAP1 Mutations in Advanced NSCLC With the TORC1/2 Inhibitor TAK-228. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, 18(4).

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