A polyclonal rabbit anti-Rac1 antibody (Abcam, Cambridge, UK), a monoclonal mouse anti-Rac1 antibody (Merck Millipore, Frankfurt, Germany), a polyclonal rabbit anti-Rac1/2/3 antibody (Cell Signaling Technology (CST), Massachusetts, USA), a polyclonal rabbit anti-WT-1 antibody (Santa-Cruz Biotechnology, Dallas, USA), a monoclonal mouse anti-synaptopodin antibody (Progen, Heidelberg, Germany), a monoclonal mouse anti-GAPDH antibody (Abcam, Cambridge, UK), a monoclonal mouse anti-β-actin antibody (Sigma-Aldrich, St Louis, CO, USA), a monoclonal mouse anti-pS6 antibody (CST), a monoclonal mouse anti-S6 antibody (CST), a polyclonal rabbit anti-pPAK1 antibody (CST), and a polyclonal rabbit anti-PAK1 antibody (CST) were purchased for immunohistochemistry or western blot (WB) analysis.
Polyclonal rabbit anti wt 1 antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Polyclonal rabbit anti-WT-1 antibody is a laboratory reagent used for the detection and study of the Wilms' tumor 1 (WT-1) protein. It is a rabbit-derived polyclonal antibody that recognizes the WT-1 protein, which is a transcription factor involved in the regulation of gene expression during development and in certain types of cancer.
Automatically generated - may contain errors
Lab products found in correlation
Monoclonal mouse anti synaptopodin antibody, by Progen Biotechnik (2 mentions)
Anti gapdh mouse monoclonal antibody, by Abcam (2 mentions)
Mouse monoclonal anti β actin antibody, by Merck Group (1 mentions)
Vector sg substrate kit, by Vector Laboratories (1 mentions)
Ez ecl kit, by Sartorius (1 mentions)
Image lab software v5, by Bio-Rad (1 mentions)
Mouse anti gapdh monoclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Chemidoc mp system, by Bio-Rad (1 mentions)
4 protocols using polyclonal rabbit anti wt 1 antibody
1
Immunohistochemistry and Western Blot Antibodies
2
Antibodies for Kidney Tissue Analysis
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The following antibodies were used in immunohistochemistry and western blot analysis: monoclonal mouse anti-Sall1 antibody (PPMX, Tokyo, Japan), polyclonal guinea pig antinephrin antibody (Progen, Heidelberg, Germany), polyclonal rabbit anti-WT1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), monoclonal mouse anti-synaptopodin antibody (Progen, Heidelberg, Germany), monoclonal mouse anti-GAPDH antibody (Abcam, Cambridge, UK), monoclonal rabbit anti-glucose-regulated protein 78 antibody (Cell Signaling Technology, Beverly, MA, USA), and polyclonal rabbit anti-cleaved caspase 3 antibody (Cell Signaling Technology, Beverly, MA, USA). Polyclonal rabbit antipodocin antibody and polyclonal rabbit anti-synaptopodin antibody have been described previously. 16
3
Podocyte and Progenitor Identification
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The rabbit anti-WT-1 polyclonal antibody (Santa Cruz Biotechnology, USA), and NCAM ( Millipore, MA, USA) were used to identify podocyte and potential PEC progenitors . Positive cells being a bluish-gray color were visualized with the Vector SG substrate kit (Vector).
4
Western Blot Analysis of WT1, NAB2, and GAPDH
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Immunoblotting was performed as previously described [44 (link)]. Briefly, membranes were blocked using 5% dry milk in TBS-T buffer, followed by incubation overnight with either of the following primary antibodies: rabbit anti-WT1 polyclonal antibody (Santa Cruz Biotechnology, Dallas, TX, USA, clone C-19, sc-192) at dilution 1:1,000 in 0.5% dry milk in TBS-T buffer, mouse anti-NAB2 monoclonal antibody (Santa Cruz Biotechnology, clone 1C4, sc-23867) at dilution 1:1,000 in 5% dry milk in TBS-T buffer, or mouse anti-GAPDH monoclonal antibody (Santa Cruz Biotechnology, clone 6C5, sc-32233) at dilution 1:3,000 in 0.5% dry milk in TBS-T buffer. The membrane was then incubated with secondary antibody (goat anti-mouse-HRP conjugate or goat anti-rabbit-HRP conjugate at dilutions 1:3,000 in 0.5% dry milk in TBS-T buffer; all secondary antibodies purchased from Bio-Rad, Hercules CA, USA) for one hour at room temperature, after which expression was analyzed using the EZ-ECL kit (Biological Industries, Kibbutz Beit Haemek, Israel) and a ChemiDoc MP system (Bio-Rad). Calculations were made using BioRad's Image Lab software v 5.2.
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