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Gramicidin

Manufactured by Thermo Fisher Scientific

Gramicidin is a type of antibiotic compound isolated from certain Bacillus bacterial species. It functions as an ionophore, facilitating the transport of monovalent cations such as sodium and potassium across cell membranes. Gramicidin is commonly used in research and laboratory settings, but a detailed description of its intended use is not available within the scope of this request.

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2 protocols using gramicidin

1

Signaling Pathway Modulation Protocol

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Brefeldin A and rapamycin was purchased from LC Labs. Golgicide A was from Sigma Aldrich. Gramicidin was from Molecular Probes. Nigericin, ruxolitinib, and tofacitinib were from Invivogen. Vinblastine was from Tocris. Human recombinant IFNβ and IFNγ were obtained from Millipore.
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2

Preparation of Pharmacological Agents

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IL-1β was purchased from R&D systems (Minneapolis, MN). Bicuculline, GABA, bumetanide and RTX were obtained from Sigma (St. Louis, MO). Muscimol was purchased from Tocris (UK). Gramicidin was purchased from Molecular Probes (ThermoFisher Scientific). IL-1β and Bicuculline were dissolved in sterile saline, and bumetanide was dissolved in sterile saline containing 70% DMSO. Gramicidin was dissolved in 100% DMSO. RTX was dissolved in a mixture containing 10% ethanol and 10% Tween 80 in sterile saline.
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