Ptp inhibitor 3 cas 29936 81 0

SC‐60 was synthesized by Hinova Pharmaceuticals Inc. (Chengdu, China), the MW of SC‐60 is 473, and its structure and solubility are described in Fig. S1. For in vitro studies, SC‐60 at various concentrations was dissolved in dimethyl sulfoxide (DMSO) and added to cells in Dulbecco's modified Eagle's medium (Invitrogen, Carlsbad, CA, USA). The final DMSO concentration was 0.1% after addition to the medium. For in vivo studies, SC‐60 was dissolved in 50% (v/v) propylene glycol and 50% (v/v) Solutol^® HS‐15. The SHP‐1 inhibitor PTP inhibitor III (CAS 29936‐81‐0) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Plasmids of human wild‐type STAT3 were encoded by pCMV6 vector with myc‐tag. The mutant SHP‐1 constructs (DN1 and D61A) have been generated to mimic the open‐form structure of SHP‐1 as previously described (Tai et al., 2014b). Antibodies for immunoblotting such as p‐VEGFR2, VEGFR2, p‐PDGFRβ, PDGFβ, p‐JAK1, JAK1, p‐JAK2, JAK2, p‐SRC, SRC, p‐STAT3, STAT3, and survivin were from Cell Signaling (Danvers, MA, USA). SHP‐1, cyclin D1, and Mcl‐1 antibodies were purchased from Abcam (Cambridge, MA, USA). Other antibodies such as poly (ADP‐ribose) polymerase (PARP) and cleaved caspase 3 were obtained from Cell Signaling Technology.

PH and Sor were purchased from Sigma-Aldrich (USA) and Selleck Chemicals LLC (Houston, TX). The SHP-1 inhibitor and PTP inhibitor III (CAS 29936-81-0) were purchased from Cayman Chemical (Ann Arbor, MI, USA). Smart-pool siRNA, including control (D-001810-10), SHP-1 (PTPN6) were all obtained from Dharmacon Inc. (Chicago, IL). The mutant SHP-1 constructs (DN1 and D61A) have been generated to mimic the open-form structure of SHP-1 as previously described [11 (link)]. Antibodies for immunoblotting such as p-STAT3, STAT3, survivin, p-Akt, Akt, p44/42 MAPK (Erk1/2) (Thr202/Tyr204), ERK, p-VEGFR2, VEGFR2, p- mTOR, mTOR, p-JAK2, JAK2, poly (ADP-ribose) polymerase (PARP) and cleaved caspase 3 were ordered from Cell Signaling Technology (Danvers, MA, USA). SHP-1, cyclin D1, Mcl-1, Ki67, b-actin antibodies were purchased from Abcam (Cambridge, MA, USA). BCA Protein Assay Kit was purchased from Pierce (Rockford, IL, USA).