Rat blood

Human liver microsomes (pooled from 20 donors, mixed gender) and rat liver microsomes (Sprague–Dawley, male) were purchased from Corning (Amsterdam, The Netherlands). Human plasma (pooled from 6 donors, mixed gender) were purchased from Zen-Bio, Inc (NC, USA). Rat plasma (Sprague–Dawley) was purchased from Innovative Research Inc. (MI, USA). For the rat samples further information on number of animals used to create the samples (liver microsomes and plasma) or on gender (plasma) was not provided by the provider. Rat blood was purchased from BioIVT (West Sussex, UK). Recombinant human AChE was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA), and the enzyme was stabilized with 1 mg/ml BSA.

Profenofos, 4-bromo-2-chlorophenol (BCP), bovine serum albumin (BSA), reduced nicotinamide adenine dinucleotide phosphate (NADPH), 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) and acetylthiocholine iodide (ATC) were purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands). Magnesium chloride hexahydrate (MgCl₂⋅6H₂O), trifluoroacetic acid (TFA), dimethylsulfoxide (DMSO), and calcium chloride dihydrate (CaCl₂⋅2H₂O) were purchased from VWR International (Amsterdam, The Netherlands). Acetonitrile (ACN, UPLC/MS grade) was purchased from Biosolve (Valkenswaard, The Netherlands). Human liver microsomes and cytosol (pooled from 150 human donors, mixed gender), rat liver microsomes and rat liver cytosol (pooled from 20 male Sprague–Dawley rats) were purchased from Corning (Amsterdam, The Netherlands). Recombinant human acetylcholinesterase was purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands). Rat blood was purchased from BioIVT (Westbury, USA), human and rat plasma were purchased from Innovate (New York, USA).