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Milliplex map 32 plex mouse cytokine chemokine panel

Manufactured by Merck Group

The MILLIPLEX MAP 32-plex mouse cytokine/chemokine panel is a multiplex assay that allows for the simultaneous quantification of 32 different mouse cytokines and chemokines in a single sample. The panel utilizes Luminex technology to detect and measure the levels of these analytes.

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2 protocols using milliplex map 32 plex mouse cytokine chemokine panel

1

Monitoring Cytokine Levels in Murine Infection

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Heparinized blood/serum was collected at 0, 6, 12, and 24 h post-infection as described above. Mouse IFN-a2/4 Platinum ELISA (eBioscience, San Diego, CA), mouse IFN-b VeriKine ELISA (PBL Assay Science, Piscataway, NJ), and mouse IL-12 (p70) as well as human/mouse TGF-b1 ELISA Ready-SET-Go (Invitrogen, Carlsbad, CA) kits were used in these studies according to the manufacturer’s instructions. Absorbance was read at 450 nm using a SpectraMax M2 Microplate Reader equipped with SoftMax Pro (v5.3b12) software (Molecular Devices). Sera were also analyzed using a MILLIPLEX MAP 32-plex mouse cytokine/chemokine panel (EMD Millipore, Billerica, MA) and analyzed on a Luminex MAGPIX System with xPONENT (v4.2) software (Luminex, Austin, TX).
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2

Monitoring Cytokine Levels in Murine Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Heparinized blood/serum was collected at 0, 6, 12, and 24 h post-infection as described above. Mouse IFN-a2/4 Platinum ELISA (eBioscience, San Diego, CA), mouse IFN-b VeriKine ELISA (PBL Assay Science, Piscataway, NJ), and mouse IL-12 (p70) as well as human/mouse TGF-b1 ELISA Ready-SET-Go (Invitrogen, Carlsbad, CA) kits were used in these studies according to the manufacturer’s instructions. Absorbance was read at 450 nm using a SpectraMax M2 Microplate Reader equipped with SoftMax Pro (v5.3b12) software (Molecular Devices). Sera were also analyzed using a MILLIPLEX MAP 32-plex mouse cytokine/chemokine panel (EMD Millipore, Billerica, MA) and analyzed on a Luminex MAGPIX System with xPONENT (v4.2) software (Luminex, Austin, TX).
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