Tbp ab818

Manufactured by Abcam

TBP (ab818) is a lab equipment product offered by Abcam. It is a protein that functions as a transcription factor, facilitating the recruitment of RNA polymerase to gene promoters.

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Lab products found in correlation

Lps l3129, by Merck Group (1 mentions) Tubulin sc 5286, by Santa Cruz Biotechnology (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Ccd 986sk, by American Type Culture Collection (1 mentions) Secondary antibodies anti mouse sc 516102 and anti rabbit sc 2357, by Santa Cruz Biotechnology (1 mentions) Il 1β, by Cell Signaling Technology (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions)

2 protocols using tbp ab818

Reagents and Antibodies for Cell Assays

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LPS (L3129) and Wedelolactone (W4016) were purchased from Sigma. U0126 (HY-12031), SP600125 (HY-12041), SB203580 (HY-10256) and LY294002 (HY-10108) were obtained from MCE. Anti-TRIM59 (HPA017750, Sigma), Sp1 (CSB-PA004135, CusAb), Nrf1 (8052, CST, for Western blot; 12,936–1-AP, Proteintech, for CHIP array), TBP (ab818, Abcam) and Tubulin (sc-5286, Santa Cruz) antibodies were purchased as indicated.

An Y., Ni Y., Xu Z., Shi S., He J., Liu Y., Deng K.Y., Fu M., Jiang M, & Xin H.B. (2019). TRIM59 expression is regulated by Sp1 and Nrf1 in LPS-activated macrophages through JNK signaling pathway. Cellular signalling, 67, 109522.

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Fibroblast Cell Line Activation and Inflammation Signaling

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The human skin fibroblast cell line CCD-986Sk was purchased from the ATCC (CRL-1947). Iscove’s modified Dulbecco’s medium (13390), fetal bovine serum (FBS; 12003C), LPS (L2630), and MSM (1437600) were purchased from Sigma-Aldrich (Merck KGaA, St. Louis, MO). Penicillin–streptomycin solution and trypsin–EDTA (0.05%) were purchased from Gibco (Thermo Fisher Scientific, Waltham, MA). Primary antibodies specific for TLR4 (sc-293072), p-Iκ-Bα (sc-8404), COX-1 (sc-19998), COX-2 (sc-47778), β-actin (sc-47778), and secondary antibodies (anti-mouse (sc-516102) and anti-rabbit (sc-2357)) were obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX). Primary antibodies specific for NF-κB (#8242), Iκ-Bα (#9242), p-IKKα/β (#9958), pERK (#9101), ERK (#9102), p-p38 (#4511), p38 (#8690), and IL-1β (#12703) were purchased from Cell Signaling Technology, Inc. (Beverly, MA). The PKC-α (ab179523), p-PKC-α (ab59411), IL-6 (ab6672), and TBP (ab818) antibodies were obtained from Abcam (Cambridge, MA). A primary antibody specific for iNOS (NB300-650) was purchased from Novus Biologicals (Littleton, CO). NTS was obtained from NaraBio (Gunsan, Korea).

Sp N., Kang D.Y., Kim H.D., Rugamba A., Jo E.S., Park J.C., Bae S.W., Lee J.M, & Jang K.J. (2021). Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts. Life, 11(5), 427.

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