The RT-PCR products were cloned into pCR4 TOPO vectors (Invitrogen, Carlsbad, CA), following the manufacturer’s procedures (Invitrogen, Carlsbad, CA). DNA from colonies was amplified using the 5’utrF and e3R primers covering cDNA regions of exons 1 and 2. The PCR products were visualized by QIAxcel (QIAGEN), purified using ExoSAP-IT (Affymetrix) and then subjected to direct DNA sequencing analysis using primers M13F and M13R (BigDye Terminator v3.1 Cycle Sequencing Kit and 3730 DNA Analyzer, Applied Biosystems, Foster City, CA).
Bigdye terminator v3.1 cycle sequencing kit and 3730 dna analyzer
Manufactured by Thermo Fisher Scientific
The BigDye Terminator v3.1 Cycle Sequencing Kit is a reagent kit used for DNA sequencing. The 3730 DNA Analyzer is an instrument used for automated DNA sequence analysis. Both products are designed for use in molecular biology and genetics research applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bigdye terminator v3.1 cycle sequencing kit and 3730 dna analyzer
1
Cloning and Sequencing RT-PCR Products
2
Cloning and Sequencing of RT-PCR Products
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