THP-1 monocytes maintained in the RPMI medium containing 10% fetal bovine serum (FBS) were differentiated into macrophages with the treatment of 100 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) for 48 hours, followed by incubation in the PMA-free medium for 24 hours. For the M1 polarization, THP-1 monocytes were treated with 100 nM PMA for 48 hours and then further treated with 20 ng/ml of recombinant human interferon-γ (IFN-γ) and 100 ng/ml of lipopolysaccharides (LPS) (Invitrogen) for another 48 hours to generate THP-1(LPS+IFN-γ) or with 20 ng/ml recombinant human interleukin 13 (IL-13) (Peprotech) and 20 ng/ml of recombinant human IL-4 (Peprotech) for another 48 hours to generate THP-1(IL-4+13).
Recombinant human interferon γ ifn γ
Manufactured by Thermo Fisher Scientific
Recombinant human interferon-γ (IFN-γ) is a cytokine produced by recombinant DNA technology. IFN-γ is a protein that plays a key role in immune system regulation and various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant human il 4, by Thermo Fisher Scientific (2 mentions)
Interleukin 4 (il 4), by Thermo Fisher Scientific (2 mentions)
Lipopolysaccharides (lps), by Thermo Fisher Scientific (2 mentions)
Ifn γ, by Thermo Fisher Scientific (2 mentions)
Recombinant human interleukin 13 il 13, by Thermo Fisher Scientific (2 mentions)
2 protocols using recombinant human interferon γ ifn γ
1
Differentiation and Polarization of THP-1 Monocytes
2
Differentiation and Polarization of THP-1 Monocytes
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THP-1 monocytes maintained in the RPMI medium containing 10% fetal bovine serum (FBS) were differentiated into macrophages with the treatment of 100 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) for 48 hours, followed by incubation in the PMA-free medium for 24 hours. For the M1 polarization, THP-1 monocytes were treated with 100 nM PMA for 48 hours and then further treated with 20 ng/ml of recombinant human interferon-γ (IFN-γ) and 100 ng/ml of lipopolysaccharides (LPS) (Invitrogen) for another 48 hours to generate THP-1(LPS+IFN-γ) or with 20 ng/ml recombinant human interleukin 13 (IL-13) (Peprotech) and 20 ng/ml of recombinant human IL-4 (Peprotech) for another 48 hours to generate THP-1(IL-4+13).
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