Tail vein blood samples were allowed to clot on ice and then centrifuged for 10 min at 10,000 × g. Serum samples were stored at −80°C until analyzed. Serum and liver triglycerides (TG) and cholesterol were measured according to manufacturer’s instructions using an Infinity kit (Thermo Fisher Scientific, Middletown, VA). Non-esterified free fatty acids (NEFA) were measured using a Wako kit (Wako Chemicals, Richmond, VA). Serum β-hydroxybutyrate (ketone) concentrations were measured with a StanBio Liquicolor kit (StanBio Laboratory, Boerne, TX). Serum insulin (Crystal Chem, Elk Grove Village, IL; cat # 90080), testosterone (ALPCO, Salem, NH; cat# 55-TESMS-E01), IL-1β, IL-6, TNF-α, MCP-1/CCL2 (all from R&D Systems, Minneapolis, MN; cat # DY401-05, DY406-05, DY410-05, DY479-05) levels were measured by ELISA according to manufacturer’s instructions.
Stanbio liquicolor kit
Manufactured by EKF Diagnostics
The StanBio Liquicolor kit is a laboratory diagnostic product designed for the quantitative determination of various analytes in biological samples. The kit provides reagents and instructions for conducting specific chemical tests, but its detailed functionality and intended use are not included in this factual description.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using stanbio liquicolor kit
1
Serum and Liver Lipid Profiling
2
Serum Biomarker Analysis in Animal Study
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Tail vein blood samples were allowed to clot on ice and then centrifuged for 10 min at 10,000g. Serum samples were stored at −80°C until analyzed. Serum and liver triglycerides (TG) and cholesterol were measured according to manufacturer's instructions using an Infinity kit (Thermo Fisher Scientific, Middletown, VA). Non‐esterified free fatty acids (NEFA) were measured using a Wako kit (Wako Chemicals, Richmond, VA). Serum β‐hydroxybutyrate (ketone) concentrations were measured with a StanBio Liquicolor kit (StanBio Laboratory, Boerne, TX). Serum insulin (Crystal Chem, Elk Grove Village, IL; cat # 90080), testosterone (ALPCO, Salem, NH; cat# 55‐TESMS‐E01), IL‐1β, IL‐6, TNF‐α, MCP‐1/CCL2 (all from R&D Systems, Minneapolis, MN; cat # DY401‐05, DY406‐05, DY410‐05, DY479‐05) levels were measured by ELISA according to manufacturer's instructions.
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