100 mg of both extracts were dissolved in 5 ml of ethanol–water at a ratio of 80:20 v/v, then the mixtures were ultrasonicated at 25 °C for 25 min at 60% duty cycles. After this step, the mixtures were centrifuged at 7500 rpm for 15 min. The supernatant of the extracts was treated with charcoal to remove pigments removal and then evaporated in vacuo. The dried extracts were mixed with 1 ml of HPLC methanol by shaking, and the suspensions were passed through a 2.5 μm filter and stored at 4 °C until analysis. The extracts were injected into the HPLC system under optimal conditions. HPLC was performed using a Shimadzu 10 AV-LC, and peaks were detected and monitored using a UV–Vis 10AV-SPD spectrophotometer (Shimadzu, Kyoto, Japan). In addition, peaks found in the extracts were detected by comparison with the suspensions of the standard materials.
10 av lc
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu 10 AV-LC is a liquid chromatography system designed for analytical and preparative applications. It features high-precision solvent delivery, a reliable autosampler, and a sensitive UV-Vis detector. The core function of the 10 AV-LC is to provide accurate and reproducible separation, identification, and quantification of chemical compounds in liquid samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using 10 av lc
1
Optimized Extraction and HPLC Analysis
2
Liquid Chromatography Analysis of H. tuberosus Powder
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To determine the main components of H. tuberosus powder samples, liquid chromatography using a Shimadzu 10 AV-LC equipped with a binary distribution pump model (LC-10A Shimadzu) was used. The eluted peaks were monitored by a Shimadzu SPD 10Avp detector and the data were reported on Shimpack C-R8A (Shimadzu, Koyota, Japan).
3
Mycotoxin Detection in Food Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five grams of the grounded samples were extracted with 20 ml chloroform. The extract was filtered through Whatman paper, evaporated to dryness, and re-dissolved in 1 ml chloroform (Kumar et al. 2010 ). The chloroform extract was evaporated to dryness and re-dissolved in deionized water for detection of mycotoxins (Ochratoxin A, Fumonisins, Patulin and Aflatoxin B1, B2, G1, G2) using high performance liquid chromatography (HPLC) Shimadzu 10AV-LC equipped with binary delivery pump model LC-10A Shimadzu. 100 μm was injected into HPLC sampler and deionized water: methanol (60 :40) was used as mobile phase at a flow rate of 1 ml / min, Mycotoxin were detected with UV detector at a wavelength of 365 nm, (Table 2) showed Retention time for each one (Akiyama 1999; Stroka et al. 2000) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!