Lipin 1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Lipin-1 is an enzyme that plays a role in lipid metabolism. It catalyzes the conversion of phosphatidate to diacylglycerol, a key step in the synthesis of triglycerides and phospholipids. Lipin-1 is involved in the regulation of lipid homeostasis and adipocyte differentiation.

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Lab products found in correlation

2 protocols using lipin 1

Western Blot Analysis of Muscle Proteins

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Skeletal muscle was lysed in radio-immunoprecipitation assay (RIPA) buffer supplemented with protease and phosphatase inhibitors. Matched protein quantities were separated by SDS-PAGE and transferred to PVDF membranes. Membranes were blocked in 3% skim milk for 2 h and then incubated with primary antibody overnight at 4 °C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech), Total OXPHOS Rodent WB Antibody Cocktail (MitoSciences), pan 14-3-3 (Santa Cruz), phospho-T172 AMPKalpha (Cell Signaling), Lipin-1 (Santa-Cruz Biotech) and total AMPKalpha (Cell Signaling). After incubation with primary antibodies, membranes were washed and probed with their respective HRP-conjugate secondary anti mouse or anti rabbit (Biorad) antibodies in 3% skim milk for 2 h at room temperature, then visualised with enhanced chemiluminescent substrate (Pierce). Approximated molecular weights of proteins were determined from a co-resolved molecular weight standard (BioRad, #1610374). Image Lab Software (Bio-Rad) was used to perform densitometry analyses, and all quantification results were normalized to their respective loading control or total protein.

Zhuang A., Yang C., Liu Y., Tan Y., Bond S.T., Walker S., Sikora T., Laskowski A., Sharma A., de Haan J.B., Meikle P.J., Shimizu T., Coughlan M.T., Calkin A.C, & Drew B.G. (2021). SOD2 in skeletal muscle: New insights from an inducible deletion model. Redox Biology, 47, 102135.

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Evaluating Lipid Metabolism Markers

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Dulbecco’s modified Eagle’s medium (DMEM) with low glucose, penicillin–streptomycin, and fetal bovine serum (FBS) were purchased from Gibco BRL (Grand Island, NY, USA). Palmitic acid was purchased from Sigma-Aldrich (St. Louis, MO, USA). The Lieber-DeCarli alcohol liquid diet was obtained from Bio-Serv (Frenchtown, NJ, USA). The antibodies of C/EBPα, SREBP1, Lipin-1, p-IκBα, and NF-κB were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). GAPDH, β-actin, p-ACC, and PPARγ antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The antibodies of HSL, ATGL, and CPT1β were obtained from Abcam plc. (Hills Road, Cambridge, UK).

Park W.Y., Song G., Noh J.H., Kim T., Kim J.J., Hong S., Park J, & Um J.Y. (2021). Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis. Nutrients, 13(12), 4448.

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