Anti ssea1

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anti-SSEA1 is a laboratory reagent used for the detection and identification of the SSEA1 (stage-specific embryonic antigen-1) marker. SSEA1 is a carbohydrate epitope expressed on the surface of undifferentiated embryonic stem cells and other cell types. The Anti-SSEA1 reagent can be used in various cell biology and stem cell research applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti sox2, by Santa Cruz Biotechnology (2 mentions) Goat anti rabbit igg h l hrp, by Abcam (2 mentions) Anti nanog, by Abcam (1 mentions) Anti oct4, by Abcam (1 mentions) Anti klf4, by Santa Cruz Biotechnology (1 mentions) Vectashield mounting medium with dapi, by Vector Laboratories (1 mentions) Anti ssea 1, by Bioss Antibodies (1 mentions) Eclipse 80i, by Nikon (1 mentions) Sc 5279, by Santa Cruz Biotechnology (1 mentions) 1x71s1f fluorescence microscope, by Olympus (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Ab18207, by Abcam (1 mentions) Anti nestin, by Abcam (1 mentions) Anti oct3 4, by Santa Cruz Biotechnology (1 mentions) Anti mouse igg hrp, by Abcam (1 mentions) Anti zfp207, by Santa Cruz Biotechnology (1 mentions) Ab3623, by Merck Group (1 mentions) Goat anti rabbit igg af568, by Thermo Fisher Scientific (1 mentions) Anti nanog, by Santa Cruz Biotechnology (1 mentions) Anti tuj1, by Abcam (1 mentions)

Spelling variants of this product

Alexa Fluor 647-conjugated anti-SSEA1 (2 citations) Anti-SSEA1-eFluor 660 (2 citations) Anti-SSEA1/CD71-eFluor660 (2 citations)

4 protocols using anti ssea1

Immunostaining of Pluripotency Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed with 3.7% formaldehyde in PBS. After permeabilization with 0.1% or 0.2% Triton X-100 in PBS, cells were incubated with a primary antibody, followed by staining with a secondary antibody conjugated to Alexa Fluor 488 (1:500), Alexa Fluor 555 (1:500), or Alexa Fluor 647 (1:500). The following primary antibodies were used, anti-SSEA1 (1:200; eBioScience, San Diego, CA, USA), anti-NANOG (1:200; Abcam, Cambridge, UK), anti-OCT4 (1:400; Abcam), anti-SOX2 (1:200; Santa Cruz Biotechnology, Dallas, TX, USA), anti-KLF4 (1:200; Santa Cruz Biotechnology), and anti-SeV-NP (1:1,000).³³ (link) Nuclei were counterstained with DAPI using VECTASHIELD mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA).

Sano M., Nakasu A., Ohtaka M, & Nakanishi M. (2019). A Sendai Virus-Based Cytoplasmic RNA Vector as a Novel Platform for Long-Term Expression of MicroRNAs. Molecular Therapy. Methods & Clinical Development, 15, 371-382.

+ Open protocol

+ Expand

Avian Germline Stem Cell Identification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following flow cytometry, cells from a subset of positive host and control embryos were incubated for 30 minutes in blocking solution, stained with FITC conjugated anti SSEA-1 (eBiosciences), VASA (Bioss Antibodies) or unconjugated EMA-1 (DHSB, University of Iowa) antibodies at room temperature for 30 minutes, followed by staining for 10 minutes in Hoechst 33342 nuclear stain. Co-localization of anti SSEA-1, VASA or EMA-1 antibodies with PKH26 and Hoechst 33342 positive staining was visualized on an Eclipse 80i (Nikon) with a Digital Sight and DS-Fi1 camera (Nikon). The anti SSEA-1, VASA and EMA-1 antibodies have previously been shown to detect avian germline stem cells (Jung et al., 2005 (link); Nakamura et al., 2007 (link); Tsunekawa et al., 2000 (link), Bioss Antibodies website).

Imus N., Roe M., Charter S., Durrant B, & Jensen T. (2014). Transfer and Detection of Freshly Isolated or Cultured Chicken (Gallus gallus) and Exotic Species’ Embryonic Gonadal Germ Stem Cells in Host Embryos. Zoological science, 31(6), 360-368.

+ Open protocol

+ Expand

Comprehensive Characterization of Induced Pluripotent Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To fully characterize iPSCs, these cells were fixed with methanol for 20 min at −20 °C, and nonspecific receptors were blocked with 10% normal goat serum. Cells in culture plates or chamber slides were fixed for 20 min at −20 °C with methanol, and nonspecific receptors were blocked with 10% normal goat serum. Oct4, SSEA1, and Nanog were stained with specific antibodies (anti-Oct4, 1:500, Santa Cruz Biotechnology SC-5279; anti-Nanog, 1:500, Santa Cruz Biotechnology SC-293121; and anti-Dppa4, 1:500, R&D AF3674; anti-SSEA1, 1:1000, Invitrogen MA1-022), followed by goat anti-mouse antibody-conjugated Texas Red (1:2000, Invitrogen T-862). Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; Invitrogen). Immunofluorescence staining was visualized, and cells were photographed with an Olympus 1X71S1F fluorescence microscope.

Ning B., Zhao W., Qian C., Liu P., Li Q., Li W, & Wang R.F. (2017). USP26 functions as a negative regulator of cellular reprogramming by stabilising PRC1 complex components. Nature Communications, 8, 349.

+ Open protocol

+ Expand

Western Blot and Immunofluorescence Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following commercially available antibodies were used at the indicated concentrations for western blot: Anti‐ZFP207 (Santa Cruz Biotechnology, sc‐271943, 1:500), Anti–β‐ACTIN (Sigma‐Aldrich, A5441, 1:2,500), Anti‐OCT3/4 (Santa Cruz Biotechnology, sc8628, 1:2,500), Anti‐Nanog (Santa Cruz Biotechnology, sc‐374001, 1:1,000), Anti‐Sox2 (Santa Cruz Biotechnology, sc‐398254, 1:1,000), Anti‐SFRS11 antibody (Abcam, ab196801, 1:2,000), Goat Anti‐Rabbit IgG H&L (HRP) (Abcam, ab6721, 1:5,000), Goat Anti‐Mouse IgG H&L (HRP) (Abcam, 1:1,000, ab6789), Rabbit Anti‐Goat IgG H&L (HRP) (Abcam, 1:5,000, ab6741), and Rabbit Anti‐ goat IgG (HRP) (Abcam, ab6771, 1:5,000), Anti‐Flag (Sigma, F3165, 1:1,000). In all western blots, β‐ACTIN was used as a loading control (Sigma, A5441, 1:2,500). For IF staining, we used Anti‐SSEA1 (Invitrogen, MA5‐17042, 1:250), Anti‐Nestin (Abcam, ab81462, 1:50), Anti‐Tuj1 (Abcam, ab18207, 1:200), Anti‐Caspase 3 Antibody, active (cleaved) form (MERK, AB3623, 1:100), Goat anti‐mouse IgG AF488 (Invitrogen, A11029, 1:1,000), Goat anti‐rabbit IgG AF568 (Invitrogen, A11011, 1:1,000), Anti‐Mouse IgG HRP (Abcam, ab6789, 1:1,000), and Donkey Anti‐Goat IgG AF594 (Invitrogen, A11058, 1:250).

Malla S., Prasad Bhattarai D., Groza P., Melguizo‐Sanchis D., Atanasoai I., Martinez‐Gamero C., Román Á., Zhu D., Lee D., Kutter C, & Aguilo F. (2022). ZFP207 sustains pluripotency by coordinating OCT4 stability, alternative splicing and RNA export. EMBO Reports, 23(3), e53191.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!