Caov 3

Normal ovarian epithelial cells IOSE80 and human ovarian cancer cell lines Caov-3, A2780, SKOV3, and CoC1 (COBIOER, Nanjing, China) were resuspended in calf serum-free RPMI1640 (PM150110, Procell Life Science & Technology, Wuhan, China). The cells were centrifuged at 1000 rpm for five min and incubated in cell medium with 15% fetal bovine serum (FBS) at 5% CO₂ and room temperature. Then the Caov-3 cells and SKOV3 cells at logarithmic phase were seeded onto 6-well plates. When confluence reached 30%~60%, cells were transfected with short interfering RNA (si)-LINC00494, overexpression (oe)-LINC00494, oe-NFκB1, si-NFκB1, and oe-FBXO32 (Shanghai GenePharma Co, Ltd, Shanghai, China) using Lipofectamine 2000 reagents (#11669-027, Invitrogen, USA).

A2780, Caov3, and SKOV3 cells (CoBioer, China) were incubated in RPMI 1640 supplemented with 10% fetal bovine serum (Gibco, USA). OVCAR3 cells (CoBioer, China) were cultured in medium containing RPMI 1640, 10% fetal bovine serum, and 1% insulin (Sigma, China).