Lps tlrl 3pelps

Manufactured by InvivoGen

Sourced in United States

LPS (tlrl-3pelps) is a laboratory product that serves as a source of lipopolysaccharide (LPS). LPS is a major component of the outer membrane of Gram-negative bacteria and is widely used in research applications.

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Lab products found in correlation

Anti phospho iκbα, by Cell Signaling Technology (1 mentions) Culture media and supplements, by Thermo Fisher Scientific (1 mentions) Allstars neg sirna af 488, by Qiagen (1 mentions) On targetplus non targeting control pool, by Horizon Discovery (1 mentions) Anti iκbα, by Cell Signaling Technology (1 mentions) Hiperfect transfection reagent, by Qiagen (1 mentions) Tlr3 dsrna complex inhibitor, by Merck Group (1 mentions) Emricasan, by Selleck Chemicals (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Sybr green pcr master mix, by Takara Bio (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Revertra ace qpcr rt kit, by Toyobo (1 mentions)

Close spelling variants of this product

Tlrl-3pelps

3 protocols using lps tlrl 3pelps

Innate Immune Signaling Pathways

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Culture media and supplements were purchased from Gibco. Chemical reagents and chemical inhibitors were purchased from Sigma and Calbiochem, respectively. TLR ligands, including poly I:C (tlr-pic), cl097 (tlrl-c97-5), ODN2395 (tlrl-2395), Pam3CSK4 (tlrl-pms), and LPS (tlrl-3pelps) were purchased from InvivoGen. Murine or human macrophage colony-stimulating factor (M-CSF) and ELISA Kits (TNF, IL-6, IFN-λ2/3, CCL2, CCL3, CCL5, and IP-10) was obtained from R&D Systems. Anti-phospho-TBK1 (#5483), anti-TBK1 (#3504), anti-phospho-IRF3 (#4947), anti-IRF3 (#4302), anti-phospho-IκBα (#9246), and anti-IκBα (#4814) Abs were purchased from Cell Signaling Technology. TLR3 antibody (#GTX20260) and TLR7 antibody (#NBP2-24906) were purchased from Genetex and Novus Biologicals. TLR3/dsRNA complex inhibitor (#614310) was purchased from Calbiochem Merck Millipore. The transfection reagents used were as follows: ON-TARGETplus Non-targeting Control Pool (Dharmacon, D-001810-10-50), ON-TARGETplus Human TLR3 siRNA-SMARTpool (Dharmacon, L-007745-00-0020), HiPerFect Transfection Reagent (Qiagen, 301707), human CLEC18 siRNA#1 (Qiagen, SI03231088), human CLEC18 siRNA#2 (Qiagen, SI04149502), AllStars Negative Control siRNA (Qiagen, 1027280), and AllStars Neg.siRNA AF 488 (Qiagen, 1027289).

Huang Y.L., Huang M.T., Sung P.S., Chou T.Y., Yang R.B., Yang A.S., Yu C.M., Hsu Y.W., Chang W.C, & Hsieh S.L. (2021). Endosomal TLR3 co-receptor CLEC18A enhances host immune response to viral infection. Communications Biology, 4, 229.

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LPS and Emricasan-Induced Hypothermia

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Littermates of both sexes were intraperitoneally injected with 20 mg/kg LPS (tlrl-3pelps, InvivoGen) and 2.5 mg/kg Emricasan (S7775, Selleck Chemicals). The mice were subsequently monitored for 8 h and body temperature was determined every 2 h using a rectal temperature probe.

Witt A., Goncharov T., Lee Y.M., Kist M., Dohse M., Eastham J., Dugger D., Newton K., Webster J.D, & Vucic D. (2023). XIAP deletion sensitizes mice to TNF-induced and RIP1-mediated death. Cell Death & Disease, 14(4), 262.

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Evaluation of Inflammatory Responses

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LBT (HY-N0327) was acquired from MedChemExpress Co., Ltd. (Shanghai, China). RPMI-1640 medium, penicillin, streptomycin, and fetal bovine serum were procured from Gibco-BRL (Thermo Fisher Scientific, Inc., Waltham, MA, United States). LPS (tlrl-3pelps; Escherichia coli 0111: B4), peptidoglycan (PGN) (tlrl-pgns2; Bacillus subtilis), cytosine-phosphate-guanine oligodeoxynucleotide (CpG ODNs) (tlrl-1585), and polyinosinic-polycytidylic acid sodium [Poly (I:C)] (tlrl-picw) were obtained from InvivoGen (CA. United States). Enzyme-linked immunosorbent assay (ELISA) kits for mouse IL-1β (88-7013A-76), IL-6 (88-7064-22), and TNF-α (88-7324-88) were sourced from Invitrogen Life Technologies (Shanghai, China). TRIzol reagent was supplied by Invitrogen Life Technologies (Shanghai, China). SYBR Green PCR Master Mix was provided by Takara, while the ReverTra Ace qPCR RT Kit came from Toyobo (Tokyo, Japan).

Chen Z., Su Y., Ding J., He J., Lai L, & Song Y. (2024). Lobetyolin protects mice against LPS-induced sepsis by downregulating the production of inflammatory cytokines in macrophage. Frontiers in Pharmacology, 15, 1405163.

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