Embryos were prepared as previously described (Yokomizo et al., 2012 (link)). A Zeiss LSM 710 AxioObserver inverted confocal microscope with ZEN 2011 software was used to acquire Z-projections and single optical projections. Images were processed using Fiji software (Schindelin et al., 2012 (link)). 3-dimensional reconstructions were produced using Volocity software (PerkinElmer). The following primary antibodies were used; rat anti-mouse CD31 (Mec 13.3, BD Pharmingen, San Diego, CA), rat anti-mouse CD117 (2B8, eBioscience, San Diego, CA), chicken anti-GFP (polyclonal, Thermo Fisher Scientific, Waltham, MA), rabbit anti-mouse Prox1 (AngioBio, San Diego, CA) and rabbit anti-human/mouse Runx (EPR3099, Abcam, Cambridge, MA). Secondary antibodies used were goat anti-rat Alexa Fluor 647 (Invitrogen, Carlsbad, CA), goat-anti rat Alexa Fluor 555 (Abcam), donkey anti-rat Alexa Fluor 555 (Abcam), goat anti-chicken Alexa Fluor 647 (Jackson ImmunoResearch, West Grove, PA) and goat anti-rabbit Alexa Fluor 488 (Invitrogen).
Rat anti mouse cd31 mec 13
Manufactured by BD
The Rat anti-mouse CD31 (Mec 13.3) is a laboratory equipment product used for the detection and identification of CD31, also known as PECAM-1, on mouse cells. CD31 is a transmembrane glycoprotein that is expressed on the surface of endothelial cells and plays a role in cell-cell adhesion. This product can be used in various applications, such as flow cytometry and immunohistochemistry, to study the expression and distribution of CD31 in mouse samples.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (2 mentions)
Lsm 710 axioobserver, by Zeiss (2 mentions)
Rat anti mouse cd117 2b8, by Thermo Fisher Scientific (2 mentions)
Chicken polyclonal anti gfp, by Thermo Fisher Scientific (2 mentions)
Donkey anti rat alexa fluor 555, by Abcam (2 mentions)
Goat anti rat alexa fluor 555, by Abcam (2 mentions)
Goat anti chicken alexa fluor 647, by Jackson ImmunoResearch (2 mentions)
Goat anti rat alexa fluor 647, by Thermo Fisher Scientific (2 mentions)
Volocity software, by PerkinElmer (2 mentions)
Bcl 2, by Novus Biologicals (1 mentions)
Vdac1 antibody ab15895, by Abcam (1 mentions)
Ecl system, by Cytiva (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Bak antibody, by Abcam (1 mentions)
3 protocols using rat anti mouse cd31 mec 13
1
Microscopic Imaging of Embryonic Vasculature
2
Zebrafish Embryo Imaging and Analysis
3
Western Blot Analysis of Apoptosis Markers
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Cells were lysed in 1.5x SDS buffer and the protein concentration determined using the BCA assay. 40 μg of protein of whole cell extracts was resolved by SDS-PAGE and transferred onto a PVDF membrane (Millipore). Membranes were blocked in 5 % non-fat milk in TN buffer (50 mM Tris–HCl pH 7.4, 150 mM NaCl) and incubated in the presence of the primary and then secondary antibodies in 5 % non-fat milk in TN buffer.
The Bak antibody was purchased from Abcam, Bid and tBid from R&D Systems, Bax and Bcl-XL from Santa Cruz, Bcl-2 from Novus, and Mcl-1 from Sigma. Rat anti-mouse CD31 (MEC 13.3) was from BD Bioscience (San Diego, CA). VDAC1 antibody (ab15895) was purchased from Abcam. Rabbit polyclonal anti-HIF-1α antibody (antiserum 2087) was produced and characterized in our laboratory [12 (link)]. ECL signals were normalized to either β-tubulin or ARD1 [13 (link)]. After washing in TN buffer containing 1 % Triton-X100 and then in TN buffer, immunoreactive bands were visualized with the ECL system (Amersham Biosciences).
The Bak antibody was purchased from Abcam, Bid and tBid from R&D Systems, Bax and Bcl-XL from Santa Cruz, Bcl-2 from Novus, and Mcl-1 from Sigma. Rat anti-mouse CD31 (MEC 13.3) was from BD Bioscience (San Diego, CA). VDAC1 antibody (ab15895) was purchased from Abcam. Rabbit polyclonal anti-HIF-1α antibody (antiserum 2087) was produced and characterized in our laboratory [12 (link)]. ECL signals were normalized to either β-tubulin or ARD1 [13 (link)]. After washing in TN buffer containing 1 % Triton-X100 and then in TN buffer, immunoreactive bands were visualized with the ECL system (Amersham Biosciences).
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