Tnf α

Manufactured by 4A Biotech

Sourced in China

TNF-α is a cytokine that plays a crucial role in the immune system. It is involved in the regulation of various cellular processes, including inflammation, cell proliferation, and cell death. This lab equipment is used to measure and analyze the levels of TNF-α in biological samples.

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Lab products found in correlation

Cxcl13, by R&D Systems (1 mentions) Ifn γ, by 4A Biotech (1 mentions) Il 10, by 4A Biotech (1 mentions)

3 protocols using tnf α

Inflammatory Cytokine Profiling in Mouse Intestine

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The levels of the inflammatory cytokines IL-1β, IL-6, IL-8, tumor necrosis factor-α (TNF-α) and IL-10 were investigated in the supernatant of mouse intestinal tissue with mouse IL-1β, IL-6, TNF-α and IL-10 ELISA kits (4A BIOTECH, China), respectively, according to the manufacturer’s instructions.

Ji Y.C., Sun Q., Fu C.Y., She X., Liu X.C., He Y., Ai Q., Li L.Q, & Wang Z.L. (2021). Exogenous Autoinducer-2 Rescues Intestinal Dysbiosis and Intestinal Inflammation in a Neonatal Mouse Necrotizing Enterocolitis Model. Frontiers in Cellular and Infection Microbiology, 11, 694395.

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CXCL13 and TNFα ELISA Quantification

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The concentrations of CXCL13 (R&D Systems, USA) and TNFα (4A Biotech., Ltd, China) were measured using ELISA kits according to the manufacturer’s instructions. All samples and standards were put into the corresponding wells. A biotinylated antibody working solution was added and incubated with an enzyme conjugate working solution for 30 min. The color developer was added in the dark, and the OD450 value was measured using a multifunctional enzyme analyzer (Perkin Elmer, USA).

Xie L., Wang H., Wu D., Zhang F., Chen W., Ye Y, & Hu F. (2023). CXCL13 promotes thermogenesis in mice via recruitment of M2 macrophage and inhibition of inflammation in brown adipose tissue. Frontiers in Immunology, 14, 1253766.

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Cytokine Profiling in Treated Mice

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The blood samples of the mice were collected after 1, 3, 7 and 14 days of treatment. Then, the blood samples were centrifugated at 3000 rpm/min after incubating at 37 °C for 1 h. The levels of IFN-γ (interferon-γ, 4 A Biotech, Beijing, China), TNF-α (tumor necrosis factor-α, 4 A Biotech, Beijing, China) and IL-10 (interleukin-10, 4 A Biotech, Beijing, China) in the serum were analyzed by the ELISA kits according to the manufacturer's protocols.

Li J., Luo G., Zhang C., Long S., Guo L., Yang G., Wang F., Zhang L., Shi L., Fu Y, & Zhang Y. (2022). In situ injectable hydrogel-loaded drugs induce anti-tumor immune responses in melanoma immunochemotherapy. Materials Today Bio, 14, 100238.

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