Peg400

The sEH inhibitor TPPU was synthesized at Professor Bruce Hammock’s lab (University of California, Davis). TPPU (3 mg/kg) was dissolved in 20% (vol/vol) polyethylene 400 (PEG 400, Cat#P8530, Solarbio, Beijing) [21 (link)]. The AHR inhibitor CH-223191 (10 mg/kg, HY-12684, MedChemExpress, USA) and AHR agonist FICZ (100 ug/kg, HY-12451, MedChemExpress, USA) were dissolved in corn oil and intragastrically administered as previously described [42 (link), 43 (link)]. TSPO antagonist PK-11195 (3 mg/kg, ab120378, Abcam, UK) was dissolved in 2% DMSO and 0.8% Tween and then diluted by saline. Finasteride (10 mg/kg, HY-13635, MedChemExpress, USA), a 5α-reductase inhibitor, was dissolved in corn oil. The above two regents were previously reported to block the effects of TSPO overexpression [44 (link)]. AC-5216 (1 mg/kg, HY-15527, MedChemExpress, USA) were suspended in 0.5% tragacanth gum aqueous (CAT#G9390, Solarbio, Beijing) solution for oral administration, which was reported to induce antianxiety and antidepressant effects [45 (link)].

NAR was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China), glycerol triacetate was bought from J&K Scientific Ltd. (Beijing, China), oleic acid was obtained from Tokyo Chemical Industries Co., Ltd. (Tokyo, Japan), propylene glycol dicaprylate and castor oil were purchased from Hunan Er-Kang Pharmaceutical Co., Ltd. (Changsha, China), PEG-40 hydrogenated castor oil (Cremophor RH40, CRH40), Cremophor EL35, and Kolliphor HS15 were bought from BASF SE (Ludwigshafen, Germany). Tween 80 was purchased from Sichuan Jinshan Pharmaceutical Co., Ltd. (Guangyuan, China), PEG400 was obtained from Solarbio Life Science (Beijing, China), and dexamethasone (DXMS) sodium phosphate eye drops were obtained from Xinxiang Huaqing Pharmaceutical Co., Ltd. (Xinxiang, China). The other reagents and solvents were analytical grade and high-performance liquid chromatography (HPLC) grade, respectively.