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Multisite gateway manual version d 2007

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The Multisite Gateway manual Version D, 2007 is a technical document that provides instructions and information for the operation and maintenance of the Multisite Gateway device. The manual covers the core functions and specifications of the device, but does not interpret or extrapolate on its intended use.

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Multisite Gateway (29 citations) Multisite Gateway Manual (5 citations)

2 protocols using multisite gateway manual version d 2007

1

Calcium Imaging and Optogenetics in Zebrafish

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All experiments were performed in adult (5 – 21 months old) zebrafish (Danio rerio) of both sexes. Fish were raised and kept under standard laboratory conditions (26–27°C; 13 h/11 h light/dark cycle). All experiments were approved by the Veterinary Department of the Canton Basel-Stadt (Switzerland).
Calcium imaging was performed using Tg(neuroD:GCaMP6f) fish36 (link) in a nacre background. Tg(SAGFF212C:Gal4), Tg(UAS:ArchT-GFP) fish were created from Tg(SAGFF212C:Gal4), Tg(UAS:GFP) fish and Tg(UAS:ArchT-GFP) fish by multiple crossings. Tg(SAGFF212C:Gal4), Tg(UAS:GFP) fish were created by the gene trap method described by Asakawa et al.51 (link). ArchT-GFP encodes a fusion protein consisting of the light-sensitive proton-pump archaerhodopsin-3 (Arch52 ) and GFP. Tg(UAS:ArchT-GFP) fish were generated using the Tol2Kit53 (link), involving a multisite recombination reaction (Invitrogen Multisite Gateway manual Version D, 2007) between p5E–UAS (5xUAS and E1b minimal promoter54 (link)), pME–ArchT-GFP (first-generation archaerhodopsin-3 fused to GFP52 ), and p3E–polyA as entry vectors, and pDestTol2CG2 as destination vector53 (link).
Huang K.H., Rupprecht P., Frank T., Kawakami K., Bouwmeester T, & Friedrich R.W. (2020). A virtual reality system to analyze neural activity and behavior in adult zebrafish. Nature methods, 17(3), 343-351.
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2

Calcium Imaging and Optogenetics in Zebrafish

Check if the same lab product or an alternative is used in the 5 most similar protocols
All experiments were performed in adult (5 – 21 months old) zebrafish (Danio rerio) of both sexes. Fish were raised and kept under standard laboratory conditions (26–27°C; 13 h/11 h light/dark cycle). All experiments were approved by the Veterinary Department of the Canton Basel-Stadt (Switzerland).
Calcium imaging was performed using Tg(neuroD:GCaMP6f) fish36 (link) in a nacre background. Tg(SAGFF212C:Gal4), Tg(UAS:ArchT-GFP) fish were created from Tg(SAGFF212C:Gal4), Tg(UAS:GFP) fish and Tg(UAS:ArchT-GFP) fish by multiple crossings. Tg(SAGFF212C:Gal4), Tg(UAS:GFP) fish were created by the gene trap method described by Asakawa et al.51 (link). ArchT-GFP encodes a fusion protein consisting of the light-sensitive proton-pump archaerhodopsin-3 (Arch52 ) and GFP. Tg(UAS:ArchT-GFP) fish were generated using the Tol2Kit53 (link), involving a multisite recombination reaction (Invitrogen Multisite Gateway manual Version D, 2007) between p5E–UAS (5xUAS and E1b minimal promoter54 (link)), pME–ArchT-GFP (first-generation archaerhodopsin-3 fused to GFP52 ), and p3E–polyA as entry vectors, and pDestTol2CG2 as destination vector53 (link).
Huang K.H., Rupprecht P., Frank T., Kawakami K., Bouwmeester T, & Friedrich R.W. (2020). A virtual reality system to analyze neural activity and behavior in adult zebrafish. Nature methods, 17(3), 343-351.
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