Srpk1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

SRPK1 is a serine/arginine-rich protein kinase that plays a role in the regulation of pre-mRNA splicing. It is involved in the phosphorylation of serine-arginine-rich domain-containing proteins, which are important components of the spliceosome.

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Lab products found in correlation

Gapdh, by Cell Signaling Technology (2 mentions) Ha tag, by Abcam (1 mentions) His tag, by Abcam (1 mentions)

Spelling variants of this product

Anti-SRPK1 (6 citations)

2 protocols using srpk1

Protein Expression Analysis via Western Blot

Cited 1 time

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Western blot analysis was performed as previously described [43 (link),44 (link)]. Briefly, proteins extracted from cells or tissues were separated on SDS–PAGE gels and transferred to PVDF membranes. The membranes were probed with antibodies against AKT2 (Santa Cruz, CA, USA, cat#sc-81436), CLK1 (Santa Cruz, cat#sc-515897), SRPK1 (Santa Cruz, cat#sc-100443), pRBM20 (gift from Dr. Hidehito Kuroyanagi), HA-tag (Abcam, Cambridge, UK, cat#ab137838), His-tag (Abcam, cat#ab18184), and GAPDH (Cell Signaling, MA, USA, cat#14C10), which served as a protein loading control.

Sun M., Jin Y., Zhang Y., Gregorich Z.R., Ren J., Ge Y, & Guo W. (2022). SR Protein Kinases Regulate the Splicing of Cardiomyopathy-Relevant Genes via Phosphorylation of the RSRSP Stretch in RBM20. Genes, 13(9), 1526.

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Protein Expression Profiling by Western Blot

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Western blotting analysis was performed as described previously [35] [36] . Briefly, Protein lysed from cells and tissues were separated by SDS-PAGE gel and transferred onto a PVDF membrane.
The membrane was probed with antibodies against AKT2 (Santa Cruz, CA, Cat#sc-81436), CLK1 (Santa Cruz, CA, Cat#sc-515897), SRPK1 (Santa Cruz, CA, Cat#sc-100443 ), GAPDH (Cell Signaling, MA, Cat#14C10) was served as the protein loading control.

Sun M., Jin Y., Zhu C., Zhang Y., Liss M., Gotthardt M., Ren J., Ge Y., & Guo W. (2020). RBM20 phosphorylation on serine/arginine domain is crucial to regulate pre-mRNA splicing and protein shuttling in the heart.

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