For Immunofluorescent staining, 50 MII-stage oocytes are used in each group. Cumulus cells and zona pellucida are removed from oocytes with 1 mg/ml hyaluronidase and acid Tyrode’s solution at room temperature respectively. Zona-free oocytes are then fixed for 40 min in 2% formaldehyde and permeabilized in 10 mM PBS + 0.1% Triton X-100 for an additional 40 min (25 (link)). After fixation and permeabilization, samples are blocked for 1 hr in 10 mM PBS + 0.3% bovine serum albumin (BSA) + 1% fetal calf serum prior to incubation in humidified chambers with primary (Abnova, 5-methylcytosine monoclonal antibody, clone 5MC-CD) and secondary antibodies (SantaCruz, goat anti-mouse IgM-CFL 488), overnight at 4 °C and for 1 hr at room temperature, respectively. Samples are counter-stained for DNA using TOTO-3 at 10 µg/ml and RNase to eliminate RNA staining. Images are obtained using an Olympus fluorescent microscope, with laser lines at 488 nm wavelengths (Tehran University of Medical Sciences, Department of Embryology) and then processed using Adobe Photoshop 7.0. Negative controls are run in the absence of primary antibodies.
Goat anti mouse igm cfl 488
Manufactured by Santa Cruz Biotechnology
Goat anti-mouse IgM-CFL 488 is a secondary antibody that binds to mouse IgM antibodies. It is conjugated with the fluorescent dye CFL 488, which emits green fluorescence when excited by the appropriate wavelength of light. This product can be used in various immunoassays and imaging techniques that require the detection of mouse IgM antibodies.
Automatically generated - may contain errors
2 protocols using goat anti mouse igm cfl 488
1
Immunofluorescent Staining of Oocyte 5-Methylcytosine
2
Western Blot Analysis of Bacterial Virulence Factors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The primary antibodies used for Western blot analysis were as follows: rat anti-integrin β1 clone AIIB2 (Merck, MABT409), rabbit anti-integrin α5 clone 5E18 ZooMAb (Sigma-Aldrich, ZRB1122-25UL), mouse anti-CD15s (sialyl Lewisx) (Santa Cruz, sc-32243), mouse anti-CagA (Santa Cruz, sc-28368), mouse anti-CagA phospho (Santa Cruz, sc-508), mouse anti-VacA (Santa Cruz, sc-32746), rabbit anti-p38 MAPK (Agrigo, ARG55258), rabbit anti-p38 MAPK phospho (Agrigo, ARG51850) rabbit anti-Cox-2 (Agrigo, ARG56421), goat anti-ATF-2 (Arigo, ARG63122), rabbit anti-ATF2 phospho (Agrigo, ARG42620), mouse anti-GAPDH (Agrigo, ARG10112). Furthermore, the secondary antibodies were as follows: donkey anti-goat IgG antibody (Arigo, ARG65352), goat anti-rabbit IgG (pre-adsorbed to 10 nm gold) (Abcam, ab27234), goat anti-rabbit IgG H&L (HRP) (Merck Millipore, AP132P), goat anti-mouse IgG H&L cross-adsorbed secondary antibody (Alexa Fluor 488) (Thermo Fisher, A-11001), goat anti-mouse IgG (HRP) (Biosciences, 554002) Goat anti-Rat IgG (HRP) (Thermo Fisher, 31470), goat anti-rabbit (Alexa Fluor 488) (Invitrogen A11034), goat anti-rat IgG H&L cross-adsorbed secondary antibody (Alexa Fluor 488) (Thermo Fisher, A-11006), and goat anti-mouse IgM CFL488 (Santa cruz, sc-395784).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!