Deionized water was further purified to 18 MΩ using a NANOpure II Water System (“Nanopure water”; Barnstead Thermolyne Corporation, Ramsey, MN). Polydimethylsiloxane (PDMS) was made from 184 Silicone Elastomer Base and 184 Silicone Elastomer Curing Agent (Dow Chemical Company, Midland, MI) in a 7:1 ratio and cured under vacuum at 50 °C. Sodium chloride, magnesium chloride hexahydrate, RNaseZap™, UltaPure agarose, SyberSafe DNA gel stain, and SyberGold DNA gel stain were purchased from Thermofisher Scientific (Waltham, MA). Sodium citrate was purchased from Mallinckrodt (Staines-upon-Thames, UK), and Tween 20 and 10x TBE buffer from Bio-Rad (Hercules, CA). Poly(ethylene glycol) (PEG) (MW 07435) was purchased from Sigma-Aldrich (Munich, Germany). All 3D printed structures were designed using SolidWorks 2020 Student Edition and printed using 1.75 mm PLA Filament (Hatchbox 3D, Pomona, CA) on an Original Prusa i3 MK3S+ 3-D Printer (Prusa Research, Prague, Czech Republic). All statistical data was analyzed using JMP Pro 16.
Original prusa i3 mk3s 3 d printer
Manufactured by Prusa Research
The Original Prusa i3 MK3S+ is a desktop 3D printer designed and manufactured by Prusa Research. It is capable of additive manufacturing through the fused filament fabrication (FFF) process, allowing users to create three-dimensional objects from a variety of thermoplastic materials.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Thermo Fisher Scientific (2 mentions)
Magnesium chloride hexahydrate, by Thermo Fisher Scientific (2 mentions)
Silicone elastomer base, by Dow (2 mentions)
184 silicone elastomer curing agent, by Dow (2 mentions)
Ultapure agarose, by Thermo Fisher Scientific (2 mentions)
Syber safe dna gel stain, by Thermo Fisher Scientific (2 mentions)
Sybergold dna gel stain, by Thermo Fisher Scientific (2 mentions)
Sodium citrate, by Mallinckrodt (2 mentions)
Tween 20 and 10x tbe buffer, by Bio-Rad (2 mentions)
Poly ethylene glycol peg mw 07435, by Merck Group (2 mentions)
2 protocols using original prusa i3 mk3s 3 d printer
1
Fabrication of Microfluidic Devices
2
Fabrication of Microfluidic Devices
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Deionized water was further purified to 18 MΩ using a NANOpure II Water System (“Nanopure water”; Barnstead Thermolyne Corporation, Ramsey, MN). Polydimethylsiloxane (PDMS) was made from 184 Silicone Elastomer Base and 184 Silicone Elastomer Curing Agent (Dow Chemical Company, Midland, MI) in a 7:1 ratio and cured under vacuum at 50 °C. Sodium chloride, magnesium chloride hexahydrate, RNaseZap™, UltaPure agarose, SyberSafe DNA gel stain, and SyberGold DNA gel stain were purchased from Thermofisher Scientific (Waltham, MA). Sodium citrate was purchased from Mallinckrodt (Staines-upon-Thames, UK), and Tween 20 and 10x TBE buffer from Bio-Rad (Hercules, CA). Poly(ethylene glycol) (PEG) (MW 07435) was purchased from Sigma-Aldrich (Munich, Germany). All 3D printed structures were designed using SolidWorks 2020 Student Edition and printed using 1.75 mm PLA Filament (Hatchbox 3D, Pomona, CA) on an Original Prusa i3 MK3S+ 3-D Printer (Prusa Research, Prague, Czech Republic). All statistical data was analyzed using JMP Pro 16.
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