Quantichrom creatinine assay kit assay

Urine was sampled (every 2–4 weeks) throughout the study and stored at − 80 °C. Albumin concentrations in urine were analyzed using a mouse albumin simple-step ELISA kit (Abcam, Cambridge, UK) according to manufacturer instructions. The QuantiChrom™ Creatinine Assay Kit assay was performed according to the manufacturer’s instructions (DICT-500, BioAssay Systems, Hayward, CA, USA) to establish the albumin/creatinine ratio. Serum was sampled 2–3 weeks from vena saphena (Microvette® CB 300 Serum, Sarstedt, Germany) after each cycle and before sacrifice (6 weeks after third cycle) and blood urea nitrogen (BUN) was analyzed with the Urea Nitrogen (BUN) Colorimetric Detection Kit (Invitrogen, Waltham, Massachusetts, USA) according to the manufacturer’s instructions.

Urine albumin was analyzed with the Mouse Albumin ELISA Kit (ab108792, Abcam, Cambridge, UK) according to the manufacturer’s instructions. The QuantiChrom™ Creatinine Assay Kit assay was performed according to the manufacturer’s instructions (DICT-500, BioAssay Systems, Hayward, CA, USA) to establish the albumin/creatinine ratio.