Acquity binary solvent manager

Manufactured by Waters Corporation

Sourced in United States

The Acquity Binary Solvent Manager is a high-performance liquid chromatography (HPLC) module designed to precisely deliver and mix two separate liquid solvents. It features a built-in solvent selection valve and a pair of low-pulsation, high-accuracy pumps to produce a consistent and reproducible mobile phase flow. The Acquity Binary Solvent Manager is a core component of the Acquity HPLC system.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Binary Solvent Manager (13 citations) ACQUITY UPLC Binary Solvent Manager (8 citations)

3 protocols using acquity binary solvent manager

UPLC-PDA Analysis of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The work was performed on a Waters Acquity™ UPLC system consisted
of Waters Acquity™ Binary Solvent Manager (two pumps), Sample Manager
(Autosampler) and PDA detector, managed with Waters Empower®
chromatography software version 3.0. LC separation was achieved on Acquity
UPLC® BEH C₁₈ column (50 × 2.1 mm, 1.7 µm)
protected with VanGuard™ pre-column (5 × 2.1 mm, 1.7 µm)
from Waters Inc, Milford, MA, USA. The LC setting was as follows: Mobile phase
solvents were 25mM NH₄AC 0.1% HOAC (A) and acetonitrile (B). The flow
rate was 0.6 mL/min. The gradient program consisted of 5%B (0–0.5min),
5–50%B (0.5–3.00min), 50–90%B (3.00–3.01min), 90%B
(3.01–3.50min), 90–5%B (3.50–3.51min), and 5%B
(3.51–4.00min). The column was heated at 40±5°C and
autosampler was cooled to 4±5 °C. The PDA detector was set at
261nm with a sampling rate of 40 points/sec. Injection volume was 5 µL.
The weak and strong needle wash solvents were 40% and 100% acetonitrile,
respectively. The seal wash solvent was 20% methanol.

Huang L., Cheah V., Chan D., Marzan F., Dvorak C.C., Aweeka F.T, & Long-Boyle J. (2019). Determination of melphalan in human plasma by UPLC-UV method. Cancer chemotherapy and pharmacology, 83(5), 905-910.

+ Open protocol

+ Expand

UPLC-MS/MS for Analytical Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

A Waters Acquity UPLC–MS/MS system (Waters Corp., Milford, MA, USA) consisting of an Acquity binary solvent manager (chromatographic pump), a sample manager (autosampler) and a column manager was used. The UPLC was connected to a Waters TQ‐S micro mass spectrometer with a triple quadrupole. The software programs Masslynx™ V4.1 and Targetlynx V4.1 were used for data processing.

Bahmany S., de Wit L.E., Hesselink D.A., van Gelder T., Shuker N.M., Baan C., van der Nagel B.C., Koch B.C, & de Winter B.C. (2018). Highly sensitive and rapid determination of tacrolimus in peripheral blood mononuclear cells by liquid chromatography–tandem mass spectrometry. Biomedical Chromatography, 33(1), e4416.

+ Open protocol

+ Expand

UPLC Characterization of Microsomal Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

All redissolved extracts from microsomal incubations of parent substances, with and without bioactivation, were analyzed by UPLC to characterize the level of metabolic conversion. The UPLC system used consisted of a Waters Acquity binary solvent manager, sample manager, and photodiode array detector, equipped with a Waters Acquity UPLC^® BEH C18 column (1.7 μm, 2.1 × 50 mm) and Waters Xbridge UPLC^® BEH C18 pre‐column (2.5 μm, 2.1 × 5 mm). The temperature of the column was set at 40°C and the autosampler at 10°C during the UPLC analysis. The mobile phase used for the analysis was (A) nanopure water with 0.1% trifluoroacetic acid, and (B) acetonitrile with 0.1% trifluoroacetic acid. The flow rate was set to 0.6 mL/min and the total run time of the UPLC analysis for each sample was 23 minutes. The gradient elution conditions were set as follows: 90:10 (A/B) from 0 to 12 minutes, changing to 10:90 (A/B) from 12 to 20 minutes, then to 90:10 (A/B) from 20 to 23 minutes. Before injection, samples were 100 times diluted in phosphate buffer. The injection volume for each diluted sample was 10 μL and test substances were detected at a wavelength of 270 nm (photodiode array detector; Waters Acquity UPLC^®). Calibration curves of PAH model compounds, being BaP, DBA and 3OH‐BaP, were also included in the UPLC analysis for quantification.

Kamelia L., de Haan L., Spenkelink B., Bruyneel B., Ketelslegers H.B., Boogaard P.J, & Rietjens I.M. (2019). The role of metabolism in the developmental toxicity of polycyclic aromatic hydrocarbon‐containing extracts of petroleum substances. Journal of Applied Toxicology, 40(3), 330-341.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!