Human il 1β elisa kit

Manufactured by Dakewe

Sourced in China

The Human IL-1β ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-1 beta (IL-1β) in serum, plasma, and cell culture supernatants.

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Lab products found in correlation

Microplate reader, by Bio-Rad (1 mentions) Human il 6 elisa kit, by Dakewe (1 mentions) Lps l2880, by Merck Group (1 mentions) Atp tlrl atpl, by InvivoGen (1 mentions) Mouse il 1β elisa kit, by Dakewe (1 mentions) Msu tlrl msu, by InvivoGen (1 mentions) Perfectstart green qpcr supermix, by Transgene (1 mentions) Nucleozol, by Macherey-Nagel (1 mentions) Mouse il 6 elisa kit, by Dakewe (1 mentions) Mouse tnf α elisa kit, by Dakewe (1 mentions)

4 protocols using human il 1β elisa kit

Quantification of IL-6 and IL-1β Release

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Serum-free medium from RASF samples was collected and centrifuged at 10,000g for 5 min. IL-6 release from RASFs into the culture medium was directly measured with a Human IL-6 ELISA Kit and Human IL-1β ELISA Kit (DAKEWE, Shenzhen, China) according to the manufacturer’s instruction. Optical density was determined with a Bio-Rad Microplate reader, and absorption was measured at 450 nm. A standard curve for each measurement was established using a cytokine standard provided by the ELISA Kit.

Zhai Y., Wu B., Li J., Yao X.Y., Zhu P, & Chen Z.N. (2015). CD147 promotes IKK/IκB/NF-κB pathway to resist TNF-induced apoptosis in rheumatoid arthritis synovial fibroblasts. Journal of Molecular Medicine (Berlin, Germany), 94(1), 71-82.

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Tumor-Derived Stimuli Regulate IL-1β and CCL2 in Macrophages

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To investigate which tumor‐derived stimulator triggers IL‐1β release from Mφ, the fresh TCM or boiled TCM or lactate was used to treat PBMC Mφ or THP1 Mφ for 72 h and then determined the IL‐1β level via a human IL‐1β ELISA Kit (DAKEWE, 1110122) according to the manufacturer's instructions.
To explore the CCL2 secretion, tumor cells were administrated with lactate‐primed THP1 Mφ culture medium or recombinant IL‐1β cytokine (10 ng/mL) for about 72 h, and then the CCL2 concentration in tumor supernatant was determined by a Human MCP‐1/CCL2 ELISA Kit (Biolegend, 438807) according to the manufacturer's instructions.

Xu C., Xia Y., Zhang B., Drokow E.K., Li H., Xu S., Wang Z., Wang S., Jin P., Fang T., Xiong X., Huang P., Jin N., Tan J., Zhong Q., Chen Y., Zhang Q., Fang Y., Ye F, & Gao Q. (2023). Macrophages facilitate tumor cell PD‐L1 expression via an IL‐1β‐centered loop to attenuate immune checkpoint blockade. MedComm, 4(2), e242.

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Quantification of IL-1β in Macrophage Cultures

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The concentration of IL-1β in medium, M1S or M2S was detected by Human IL-1β ELISA Kit (DKW12-1012-096, DAKEWE, China) according to the manufacture's instruction.

Zong Z., Zou J., Mao R., Ma C., Li N., Wang J., Wang X., Zhou H., Zhang L, & Shi Y. (2019). M1 Macrophages Induce PD-L1 Expression in Hepatocellular Carcinoma Cells Through IL-1β Signaling. Frontiers in Immunology, 10, 1643.

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Inflammatory Cytokine Assay Protocol

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Dulbecco’s modified Eagle medium (DMEM, high glucose, 11965092), fetal bovine serum (FBS, 10099141), Lipofectamine™ 2000 (11668019), Pierce™ BCA Protein Assay Kit (23225), and Thermo Scientific RevertAid Mix (M16325) were purchased from ThermoFisher Scientific (Waltham, MA, USA). Protease inhibitor cOmplete™ Cocktail (4693116001) was purchased from Roche (Basel, Switzerland). PerfectStart™ Green qPCR SuperMix (AQ601) was purchased from TransGen Biotech (Beijing, China). NucleoZOL (740404) was purchased from MACHEREY-NAGEL (MN, Düren, Deutschland). Mouse IL-1β ELISA kit (1210122), human IL-1β ELISA kit (1110122), mouse IL-6 ELISA kit (1210602), and mouse TNF-α ELISA kit (1217202) were purchased from DAKEWE (Shenzhen, China). LPS (L2880) was purchased from Sigma (Missouri, USA). ATP (tlrl-atpl) and MSU (tlrl-msu) were purchased from In vivoGen.

Li J., Lin H., Fan T., Huang L., Zhang X., Tai Y., Fang Y., Li Q., Zhao R., Wang P., Zhou L., Wan L., Wu Y., Zhong H., Wei C, & Yang X. (2023). BPOZ-2 is a negative regulator of the NLPR3 inflammasome contributing to SARS-CoV-2-induced hyperinflammation. Frontiers in Cellular and Infection Microbiology, 13, 1134511.

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