Paraquat and tacrolimus were purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. High glucose DMEM and FBS were obtained from HyClone; Cytiva. MTT reagent was purchased from Amresco, LLC. Rat TGF-β1 (cat. no. CSB-E04727r), SMAD3 (cat. no. CSB-EL021788RA), SMAD7 (cat. no. CSB-E09225r) and connective tissue growth factor (CTGF; cat. no. CSB-E07876r) levels were analyzed using ELISA kits purchased from Cusabio Technology LLC. BSA (cat. no. A8020), the TGF-β1 receptor type I/II dual inhibitor (LY2109761; cat. no. ab29286) and anti-SMAD3 antibody (cat. no. ab40854) were obtained from Abcam, and the anti-SMAD7 antibody (cat. no. 42-0400) was purchased from Thermo Fisher Scientific, Inc. The Cy3 conjugated Goat Anti-Rabbit IgG secondary antibody (cat. no. GB21303) was obtained from Wuhan ServiceBio Technology Co., Ltd. RNA extracting fluid (cat. no. G3013) was obtained from Wuhan ServiceBio Technology Co., Ltd. HyPure™ Molecular Biology Grade Water (cat. no. SH30538.02) was obtained from HyClone. RevertAid First Strand cDNA Synthesis kit was obtained from Thermo Fisher Scientific, Inc. FastStart Universal SYBR Green Master (Rox) was obtained from Roche Diagnostics. Primers were obtained from Wuhan ServiceBio Technology Co., Ltd.
Cy3 conjugated goat anti rabbit igg secondary antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
Cy3 conjugated Goat Anti-Rabbit IgG secondary antibody is a reagent used in immunofluorescence and other research applications. It is designed to bind to and detect rabbit primary antibodies, allowing visualization of target proteins or other biomolecules.
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2 protocols using cy3 conjugated goat anti rabbit igg secondary antibody
1
Paraquat and Tacrolimus Modulate TGF-β1 Signaling in Fibrosis
2
Immunofluorescence of Sertoli Cells
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Immunofluorescence was performed on Sertoli cells seeded on coverslips, according to a previously published protocol [37] . Briefly, cells were washed with phosphatebuffered saline (PBS) and fixed with cold methanol-acetone. Non-specific binding was blocked with normal goat serum (NGS), then primary antibodies (listed in Table 2) followed by Cy3-conjugated goat anti-rabbit IgG secondary antibody (1:200; Thermo Fischer Scientific, Rocheford, IL, USA) were used. Images were captured with epifluorescence microscope Nikon Eclipse Ni (Nikon Instech Co., Tokyo, Japan). To confirm the specifity of primary antibodies negative controls with the omission of the primary antibody were performed (not shown).
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