Anti mouse cd16 cd32 2.4g2

Manufactured by BioLegend

Anti-mouse CD16/CD32 (2.4G2) is a monoclonal antibody that binds to the mouse CD16 and CD32 receptors. It is used in flow cytometry and other immunoassays to block non-specific Fc receptor binding.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Efluor506 fvd, by Thermo Fisher Scientific (1 mentions) Lb broth, by Sangon (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Ampicillin, by Dingguo (1 mentions) Fluorescent beads, by Spherotech (1 mentions) Sytox blue dead cell stain, by Thermo Fisher Scientific (1 mentions) Canto 2, by BD (1 mentions)

2 protocols using anti mouse cd16 cd32 2.4g2

Murine Immune Cell Isolation and Phenotyping

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RPMI 1640, Dulbecco’s modified Eagle’s medium (DMEM), FBS, penicillin, and streptomycin were obtained from Invitrogen (Grand Island, NY, United States). Red blood cell (RBC) lysis buffer (Cat: C3702-500ml) was obtained from Beyotime Biotechnology (Shanghai, China). The LB broth (Cat: SD7002) was obtained from Sangon Biotech (Shanghai, China). Ampicillin (Cat: MCR003) was obtained from Dingguo Biotech (Beijing, China). The following fluorescein-conjugated anti-mouse antibodies were obtained from BD and Biolegend: FITC-CD3 (145-2C11), APC-cy7-CD8 (54-6.7), Percp-cy5.5-CD4 (GK1.5), Percp-cy5.5-CD19 (6D6), APC-CD62L (MEL-14), APC-PD-1 (29F.1A12), PE-cy7-ICOS (C398.4A), Brilliant Violet 421-CD69 (H12F3), PE-CD25 (BC96), BV421-CXCR5 (J252D4), PE-CD40L (MR1), APC-IFN-γ (XMG1.2), PE-IL4 (11B11), PE-IL10 (JES5-16E3), PE-IL2 (JES6-5H4), APC-IL21 (FFA21), APC-IL5 (TRFK5), eFlour660-IL13 (Blo13A), APC-CD45 (30-F11), and anti-mouse CD16/CD32 (2.4G2). The eFluor506-FVD (Cat: 65-0863-14) was obtained from eBioscience. The Alexa Fluor 488 anti-rabbit IgG (Cat: 4412S), Helios rabbit mAb (D8W4X), histone H3 rabbit mAb (D1H2), α-tubulin antibody (Cat: 2144S), and HRP-conjugated secondary antibodies (Cat: L3012-2) were obtained from Cell Signaling Technology.

Xie S., Wei H., Peng A., Xie A., Li J., Fang C., Shi F., Yang Q., Huang H., Xie H., Pan X., Tian X, & Huang J. (2021). Ikzf2 Regulates the Development of ICOS+ Th Cells to Mediate Immune Response in the Spleen of S. japonicum-Infected C57BL/6 Mice. Frontiers in Immunology, 12, 687919.

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Multiparameter Flow Cytometric Analysis

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Erythrocytes in 50 μL blood were lysed by ammonium chloride treatment (Gibco). 1–2 μg FcR block (anti-mouse CD16/CD32, 2.4G2, BioLegend) was added to the samples, and after 20 minutes, 1x10⁶ cells were incubated with 1 μg of anti-mouse antibodies specific for CD3 [145–2C11, Peridinin Chlorophyll Protein Complex (PerCP); BioLegend], CD4 [GK1.5, fluorescein isothiocyanate (FITC); BioLegend], CD8 [53–6.7, phycoerythrin (PE) or allophycocyanin (APC); BioLegend], TCRvβ6 (RR4–7, PE, FITC or APC; BD Biosciences), TCR-2C [first antibody: 1B2 (48 (link)), secondary antibody: APC-labeled goat anti-mouse IgG (Invitrogen)], CD45R (RA3–6B2, PE; BioLegend) or using H-2K^b:SIY multimers [Dimer X (loaded with SIY peptide following the manufacturer’s protocol), PE; BD Biosciences]. Separation of living and dead cells was performed using SYTOX Blue Dead Cell Stain (Invitrogen). For T-cell quantification, fluorescent beads (Spherotech, Lake Forest, IL) were used according to manufacturer’s specifications. Samples were analyzed using flow cytometers LSR II or Canto II and FlowJo software (BD Biosciences).

Schreiber K., Karrison T.G., Wolf S.P., Kiyotani K., Steiner M., Littmann E.R., Pamer E.G., Kammertoens T., Schreiber H, & Leisegang M. (2019). Impact of TCR diversity on the development of transplanted or chemically induced tumors. Cancer immunology research, 8(2), 192-202.

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