Isg15
ISG15 is a protein involved in the post-translational modification of other proteins. It functions as a ubiquitin-like modifier, which can be conjugated to target proteins, altering their function, interactions, or subcellular localization.
Lab products found in correlation
5 protocols using isg15
Immunoblotting Protocol for Protein Analysis
Quantitative PCR analysis of human liver
Optimized Immune Modulation Assays
Recombinant cytokines used were: IL-10 [100 ng/mL] [#200–10], IL-1β [100 ng/mL] [#200-01B], TNF [100–200 ng/mL] [#300-01A], and IFNβ [0.1–10 ng/ml] [#300-02BC] [all from PeproTech], IL12 [20 ng/mL] [#219-IL, R&D], and ISG15 [500 ng/ml] [#12729HNAE, Thermo Fisher Scientific]. JAK-inhibitors used were: filgotinib [10 µM] [GLPG0634] [#S7605, Selleckchem.com] and ruxolitinib [10 µM] [#tlrl-rux, InvivoGen]. Neutralising antibodies used were: rabbit anti-human IFN-β [2.5 µg/ml] [#500-P32B, PeproTech] and isotype control rabbit IgG [2.5 µg/ml] [#X0903, Dako Cytomation]. For details, see
RT-qPCR Analysis of Antiviral Genes
RNA Purification and qPCR for Viral Genes
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!