DA solutions were prepared daily from dopamine hydrochloride powder (Sigma) and protected from light during the experiments. 1 mM DA was used in ANF loose-patch extracellular and current-clamp recordings, 1–2 mM DA was used in ANF voltage-clamp recordings. 0.01–0.1% (w/v) sodium ascorbate was supplemented in the DA solution as an antioxidative agent, except for two ANF recordings. For control experiments, DA but not sodium ascorbate was omitted in the Control solution. In a subset of voltage-clamp recordings, 1–2 µM TTX (Tocris) was present to block APs.
Dopamine hydrochloride powder
Manufactured by Merck Group
Sourced in United States
Dopamine hydrochloride powder is a chemical compound that is used in various laboratory and research applications. It is a white, crystalline powder that is soluble in water and other polar solvents. The core function of dopamine hydrochloride powder is to serve as a chemical standard or reference material for analytical and research purposes, particularly in the fields of neuroscience, pharmacology, and biochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Kh2po4, by Merck Group (1 mentions)
Na2hpo4, by Merck Group (1 mentions)
Oxalic acid, by Merck Group (1 mentions)
Methyl violet 2b mv2b, by Merck Group (1 mentions)
Nickel nitrate, by Merck Group (1 mentions)
Tris hcl buffer, by Merck Group (1 mentions)
Medroxyprogesterone, by Merck Group (1 mentions)
3 protocols using dopamine hydrochloride powder
1
Dopamine Extracellular Recordings in ANF
2
Methyl Violet 2B and Chromotrope 2B Synthesis
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Methyl Violet 2B (MV2B) (Figure 1 ) and Chromotrope 2B were supplied by Sigma-Aldrich (St. Louis, MO, USA). Oxalic acid, nickel nitrate, dopamine hydrochloride powder, and tris–HCl buffer were also obtained from Sigma-Aldrich and used without further purification. KH2PO4 and Na2HPO4 were of high-grade quality from Merck (Berlin, Germany) and used as received. Distilled water and ethanol were the main solvents used throughout the work.
3
Freshwater Prawn Neurobehavioral Assay
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The experimental prawn was giant freshwater prawn Asahan strain. The average weight was 11.27 ± 0.97 g. The prawn was four-month old, healthy, no defect, complete body organ, and pathogen-free. Dopamine hydrochloride powder (Sigma Aldrich), medroxyprogesterone, and Estradiol kit (USA) were utilized in laboratory analysis.
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