Salmon sperm dsdna

Manufactured by Merck Group

Sourced in Slovakia, United States

Salmon sperm dsDNA is a commercial DNA product derived from the sperm of salmon. It consists of double-stranded DNA (dsDNA) and is commonly used in various laboratory applications, such as molecular biology and biochemical research.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using salmon sperm dsdna

ELISA for Anti-Nuclear Antibodies

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immulon 4HBX high binding plates (ThermoFisher, Rockford, IL) were used for ELISA assays. For ANA ELISAs, plates were coated with a 1:10 dilution of poly-L-lysine (Sigma-Aldrich, St. Louis, MO), followed by coating with salmon sperm dsDNA (Sigma-Aldrich, St. Louis, MO) or Smith/Ribonucleoprotein SmRNP (Arotec Diagnostics, Wellington, New Zealand). Plates were blocked with 4% non-fat dry milk in PBS and coated with diluted serum samples with serial double dilution. Detection of antibody subtypes was performed using the following combinations of primary and secondary detection antibodies: primary- anti-IgG-biotin (Jackson ImmunoResearch, West Grove, PA), anti-IgG2b-biotin (Southern Biotech, Birmingham, AL), anti-IgG2c-alkaline phosphatase (Southern Biotech, Birmingham, AL) and secondary- streptavidin-alkaline phosphatase (Vector Laboratories, Burlingame, CA). Plates were developed with PNPP (p-nitrophenyl phosphate, disodium salt) (ThermoFisher, Rockford, IL) substrate and quantitation was performed as previously described [50 (link)]. Anti-DNA or anti-SmRNP IgG-subclass titers were determined by calculating arbitrary units by using serial dilution of the serum from a positive animal as a standard, and were expressed as units per volume.

Soni C., Sinha I., Fasnacht M.J., Olsen N.J., Rahman Z.S, & Sinha R. (2019). Selenium supplementation suppresses immunological and serological features of lupus in B6.Sle1b mice. Autoimmunity, 52(2), 57-68.

+ Open protocol

+ Expand

Synthesis and Functionalization of GA-AgNPs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Salmon sperm dsDNA was purchased from Sigma Aldrich (Slovakia) and its 0.1 mg mL⁻¹ stock solution prepared in PB was stored at 4 °C. 4-Chloro-7-nitrobenzofurazan (NBD-Cl) was purchased from Sigma Aldrich (Slovakia) and prepared as a stock solution of 2 mg mL⁻¹ (10 mmol L⁻¹) in acetonitrile. GA-AgNPs were synthesized in our laboratory using the protocol described below. Silver nitrate, gallic acid, sodium hydroxide, ammonia, sodium hydrogen phosphate, sodium dihydrogen phosphate, and other required chemicals were obtained from Mikrochem (Slovakia). For GA-AgNPs modification, doxorubicin (DOX) was purchased from Sigma Aldrich (Slovakia) and its 1 mg mL⁻¹ stock solution was prepared in deionized water and kept in dark.

Nemčeková K., Svitková V., Sochr J., Gemeiner P, & Labuda J. (2022). Gallic acid-coated silver nanoparticles as perspective drug nanocarriers: bioanalytical study. Analytical and Bioanalytical Chemistry, 414(18), 5493-5505.

+ Open protocol

+ Expand

Electrochemical Sensing of Biomolecules

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ciprofloxacin, salmon sperm dsDNA, sodium acetate, acetic acid, uric acid, ascorbic acid, dopamine hydrochloride, NaOH, HCl, Na2HPO4, NaH2PO4•2H2O, KH2PO4, potassium ferrocyanide and potassium ferricyanide were purchased from Sigma-Aldrich (MO, USA).
Graphene nanopowder was obtained from Graphene Supermarket (USA). All solutions were prepared and diluted using double-distilled water.

Lim S.A, & Ahmed M.U. (2016). A Simple DNA-based Electrochemical Biosensor for Highly Sensitive Detection of Ciprofloxacin Using Disposable Graphene. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 32(6).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!