Total liver protein extracts were prepared using a lysis buffer containing 50 mM Tris–HCl (pH = 7.5), 137 mM NaCl, 1 mM EDTA, 1% Triton X-100, 10% glycerol, 10 mM NaF, 10 mM Na4P2O7, 1 mM Na3VO4, and protease inhibitor cocktail. The lysates were separated by SDS-PAGE and transferred to a PVDF membrane, followed by immunoblotting with primary antibodies and secondary antibodies. The following antibodies were used: anti-TBK1 (Cell Signaling Technology, 3013); anti-phospho-TBK1S172 (Cell Signaling Technology, 5483); anti-IFIT1 (Thermo Fisher, PA3-846); anti-IFIT3 (ProteinTech, 15201-1-AP); anti-ZBP1; anti-STAT1 (Cell Signaling Technology, 9172); anti-phospho-STAT1Y701 (Cell Signaling Technology, 9171); anti-HSP90 (Santa Cruz BioTech, sc-7947). TSK antibody was generated in rabbits with a synthetic peptide corresponding to mouse TSK (amino acids 317-335; CRRLVREGAYHRQPGSSPK) and affinity purified.
Anti phospho tbk1s172
Manufactured by Cell Signaling Technology
Anti-phospho-TBK1S172 is a laboratory reagent used to detect the phosphorylation of the TBK1 protein at the serine 172 residue. It is used in research applications to study the regulation and signaling of the TBK1 protein, which is involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti tbk1, by Cell Signaling Technology (2 mentions)
Anti hsp90, by Santa Cruz Biotechnology (1 mentions)
Anti ifit3, by Proteintech (1 mentions)
Anti ifit1, by Thermo Fisher Scientific (1 mentions)
Anti phospho stat1 y701, by Cell Signaling Technology (1 mentions)
Anti sting, by Cell Signaling Technology (1 mentions)
Anti cgas, by Cell Signaling Technology (1 mentions)
Anti scd2, by Santa Cruz Biotechnology (1 mentions)
Anti mavs, by Cell Signaling Technology (1 mentions)
Anti phospho sting ser365, by Cell Signaling Technology (1 mentions)
Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti phospho tbk1s172
1
Protein Extraction and Western Blot Analysis
2
Immunoblotting of Immune Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunoblotting was performed as described previously39 (link). Briefly, cytoplasmic extracts and nuclear extracts were prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagent (Thermo Fisher Scientific). The antibodies used for the immunoblot analysis were anti-SCD2 (Santa cruz), anti-cGAS (Cell signaling), anti-MAVS (Cell signaling), anti–STING (Cell signaling), anti–phosphoSTING (Ser365) (Cell signaling), anti–TBK1 (Cell signaling), anti–phosphoTBK1 (S172) (Cell signaling) and anti-Tublin (Thermo).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!