O npoe

Manufactured by Merck Group

Sourced in United States, Germany

O-NPOE is a laboratory chemical commonly used as a plasticizer in the manufacture of ion-selective electrodes. It is a colorless, viscous liquid with a high boiling point. O-NPOE helps to maintain the physical integrity and performance of ion-selective membranes in electrochemical sensors and detectors.

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Close spelling variants of this product

Ortho nitrophenyl octyl ether (o-NPOE), O-nitrophenyl octyl ether (o-NPOE) 2-nitrophenyl octyl ether (o-NPOE)

8 protocols using o npoe

Synthesis and Characterization of Mg-based Ionophores

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The reagents used for the synthesis of ionophores Mg1–Mg5 included 1,10-diaza-18-crown-6 (Synthon); BOP-Cl, diethyl methylmalonate, diethyl n-butylmalonate, adamantylamine, n-octadecylamine, n-dodecylamine, triethylamine, thionyl chloride (Sigma-Aldrich) and other typical reagents and solvents.
The reaction progress and purity of products were monitored by TLC using aluminum sheets covered with silica gel 60F₂₅₄ (Merck, Kenilworth, NJ, USA). Reaction mixtures were separated using classical column (silica gel 60, 0.063–0.200 mm, Merck). Reagent grade solvents were used.
¹H NMR spectra were recorded on Varian INOVA 500 spectrometer at 500 MHz. Chemical shifts are reported in δ (ppm) units. FTIR spectra (film and KBr pellet) were taken on a Nicolet iS10 apparatus. ESI mass spectra were taken on SYNAPT G2-S HDMS (Waters, Milford, MA, USA) spectrometer using a TOF mass analyzer.
RP-18 F254S 0.25 mm modified silica gel reverse phase TLC plates (20 × 20 cm) from Merck were used for the determination of ionophore lipophilicity.
Plasticizers with known lipophilicity, namely o-NPOE, DOS, and BBPA (Sigma-Aldrich, St. Louis, MO, USA), were used as reference.

Pomećko R., Luboch E, & Jeszke M. (2021). Dipodal Tetraamide Derivatives of 1,10-Diaza-18-Crown-6 and Alkylmalonic Acids—Synthesis and Use as Ionophores in Ion Selective Membrane Electrodes. Sensors (Basel, Switzerland), 21(15), 4984.

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Potentiometric Sensing Electrode Preparation

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Reagents used for electrode preparation and potentiometric measurements included tetrahydrofuran (≥99.9%), o-NPOE (≥99%) (Sigma-Aldrich); sodium chloride (≥99%), potassium chloride (≥99%), calcium chloride (≥98%), (POCH), magnesium chloride (≥99%) (Sigma-Aldrich), poly(vinyl chloride) (PVC, high molecular weight), potassium tetrakis(4-chlorophenyl)borate (KTpClPB) (≥98%) (Fluka), poly(3,4-ethylenedioxythiophene)/poly(styrenesulfonate) blend (PEDOT/PSS, used as 1.3% (w/w) dispersion in water (conductive grade) (Sigma-Aldrich).
The aqueous solutions were prepared with deionized water of conductivity below 0.1 μS/cm, obtained using a Hydro-Lab-PL reverse osmosis (RO) station.
Potentiometric tests were carried out using a 16-channel multimeter (Lawson Labs, Malvern, PA, USA), IS 561 conventional electrode bodies (Moeller S.A., Zurich, Switzerland), glassy carbon electrodes (glassy carbon disks of 1.8 mm in diameter in poly(ether ether ketone) casing were obtained from Mineral ^® (Warsaw, Poland)), silver chloride reference electrode (3M KCl) with electrolyte key (1M lithium acetate) (Philips), ORION 800500U Ross Ultra D/J Reference electrode (ThermoScientific, Waltham, MA, USA, patented composition).

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Copper and Zinc Ion Sensing Assay

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Polyvinyl chloride (PVC) of high molecular weight, 2-nitrophenyl octyl ether (o-NPOE), sodium tetrakis [3,5-bis (trifluoromethyl)phenyl] borate (NaTFPB), the copper(II) ionophore I—o-xylylene bis diisobutyldithiocarbamate (o-XBDiBDTC), potassium tetrakis(p-chlorophenyl) borate (KTCPB), the zinc ionophore I—tetra butylthiuram disulfide (TBTDS), tetrahydrofuran (THF), copper chloride, zinc chloride, phosphate buffered saline (PBS, liquid, sterile-filtered, suitable for cell culture), ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA), were purchased from Sigma Aldrich, Merck KGaA, Darmstadt, Germany. Bovine Serum Albumin (BSA), E-cadherin/CD324 Recombinant Rabbit Monoclonal Antibody, Alexa Fluor^® 647 Goat anti-Rabbit IgG secondary antibody, SlowFade^® Gold Antifade Mountant with DAPI were purchased from Thermo Fisher Scientific Inc., Waltham, MA, USA.

Sciurti E., Blasi L., Prontera C.T., Barca A., Giampetruzzi L., Verri T., Siciliano P.A, & Francioso L. (2023). TEER and Ion Selective Transwell-Integrated Sensors System for Caco-2 Cell Model. Micromachines, 14(3), 496.

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Potentiometric Sensor Fabrication

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The following
chemicals were purchased from Sigma-Aldrich: MAA, MWCNTs, barbiton
sodium salt, methanol, acetonitrile, high molecular weight PVC, o-nitrophenyl octyl ether (o-NPOE), PBAR,
PHBAR, tridodecylmethylammonium chloride (TDMAC), EGDMA, and tetradodecylammonium
tetrakis(4-chlorophenyl)borate (ETH 500). THF and benzoyl peroxide
(BPO) were bought from Fluka. The use of all other reagents, which
were of analytical quality, was without additional purification. Freshly
deionized water (18.2 M cm specific resistance) was used to make all
aqueous solutions using the Milli-Q PLUS reagent-grade water system
(Millipore, Burlington, MA, USA).

Al Shagri L.M., Kamel A.H., Abd-Rabboh H.S, & Bajaber M.A. (2022). Molecularly Imprinted Polymer Modified with an MWCNT Nanocomposite for the Fabrication of a Barbital Solid-Contact Ion-Selective Electrode. ACS Omega, 7(37), 32988-32995.

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Synthesis of PVC-Based Ion-Selective Electrodes

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High molecular weight poly(vinyl chloride) (PVC), 2-nitrophenyl octyl ether (o-NPOE), tetradodecylammonium tetrakis(4chlorophenyl)-borate (ETH 500), and dinonylnaphthalene sulfonic acid (DNNS À H + ) as a 50 wt% solution in heptane were purchased from Sigma. 4,4 0 -Biphenyldiboronic acid was purchased from J&K chemical. PAPMA with a molecular weight of ca. 3000 was purchased from Shanghai Boka Chemical. Other reagents were purchased from Sinopharm Group Co., Ltd. All chemicals were of selectophore or analytical reagent grade. Aqueous solutions were prepared with freshly deionized water (18.2 MU cm specic resistance) obtained with a Pall Cascada laboratory water system.

Li L., Li Y., Qin W, & Qian Y. (2020). Potentiometric detection of glucose based on oligomerization with a diboronic acid using polycation as an indicator. Analytical methods : advancing methods and applications, 12(36).

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Naltrexone Hydrochloride Analytical Procedures

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Pure grade of opioid antagonist medication Naltrexone hydrochloride and Naltrexone hydrochloride^® tablets (50 mg/tablet) was gained from (Intas Pharmaceuticals Limited, Ahmedabad, India). Sigma Aldrich, Hamburg, Germany, supplied various analytical chemicals and solvents, including acetone 99.9%, m ethanol 99.9%, ethanol 99.9%, tetrahydrofuran (THF) 97.0%, o-NPOE, hydrochloric acid 37%, sodium tetraphenylborate (Na-TPB) and high molecular weight PVC. Copper chloride (99.0%), aluminium nitrate nonahydrate (99.9%) and sodium hydroxide (99.9%) were obtained from BDH (Poole, UK).

Al-Mohaimeed A.M., Mostafa G.A, & El-Tohamy M.F. (2021). New Construction of Functionalized CuO/Al2O3 Nanocomposite-Based Polymeric Sensor for Potentiometric Estimation of Naltrexone Hydrochloride in Commercial Formulations. Polymers, 13(24), 4459.

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Electrochemical Sensing of Gallic Acid

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High molecular weight poly(vinyl chloride) (PVC), 2-nitrophenyl octyl ether (o-NPOE), tridodecylmethylammonium chloride (TDMAC), tetrahydrofuran (THF) and gallic acid (GA) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Potassium permanganate, tartaric acid, sodium tartrate, sodium chloride, lithium acetate and ethanol were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). A stock solution of 0.1 M for gallic acid was prepared with the model wine solution (12% vol ethanol, 4 g/L tartaric acid, pH 3.6). A 0.1 M Potassium permanganate was prepared daily and stored in the dark. Other aqueous solutions were prepared by dissolving the appropriate salts in the freshly de-ionized water (18.2 MΩ cm specific resistance) obtained with a Pall Cascada laboratory water system.

Sun Z., Zhang Y., Xu X., Wang M, & Kou L. (2019). Determination of the Total Phenolic Content in Wine Samples Using Potentiometric Method Based on Permanganate Ion as an Indicator. Molecules, 24(18), 3279.

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Solid-Contact Electrodes with Nanomaterials

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The membrane components—potassium ionophore I (Valinomycin), lipophilic salt– potassium tetrakis(4-chlorophenyl)borate (KTpClPB), 2-nitrophenyl octyl ether (o-NPOE), and poly(vinyl chloride) (PVC)—were purchased from Sigma–Aldrich and dissolved in tetrahydrofuran (Sigma–Aldrich, Saint Louis, MO, USA).
Three different nanomaterials were tested as an intermediate layer in the construction of solid-contact electrodes. Dispersions of material in THF contained 5 mg/mL of the following materials: eCNF, eCNF-Co and eCNF/CNT-NiCo. The materials were prepared according to the method described by Zambrzycki [17 (link)]. These materials were studied in previous work, where their preparation was fully described [14 (link)]. Poly(3-octylthiophene-2,5-diyl) (POT) was purchased from Sigma–Aldrich.
Potassium chloride (KCl) was purchased from POCH (Gliwice, Poland), and solutions of K⁺ ions with concentrations from 10⁻⁷ to 10⁻¹ M were used for potentiometry, chronopotentiometry, and EIS measurements.

Niemiec B., Piech R, & Paczosa-Bator B. (2022). Modification of Carbon Nanomaterials by Association with Poly(3-octylthiophene-2,5-diyl) as a Method of Improving the Solid-Contact Layer in Ion-Selective Electrodes. Membranes, 12(12), 1275.

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