Log In Sign up
The largest database of trusted experimental protocols

Pf364402

Manufactured by Merck Group
Visit Supplier
Sourced in United States

PF364402 is a lab equipment product from Merck Group. It is a compact and versatile device designed for laboratory use. The core function of this product is to perform specific tasks required in a research or testing environment. However, a detailed description of its intended use or capabilities cannot be provided in an unbiased and factual manner without the risk of interpretation or extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

Tert butyl hydroperoxide tbhp, by Thermo Fisher Scientific (1 mentions) Ct26 cells, by Merck Group (1 mentions) Tnf α, by BioLegend (1 mentions) Interleukin 6 (il 6), by BioLegend (1 mentions) Il 1β, by BioLegend (1 mentions) Ct26 cells, by American Type Culture Collection (1 mentions)

3 protocols using pf364402

1

Evaluating Cell Death Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tert-butyl hydroperoxide (t-BHP, #A13926) was purchased from Alfa Aesar, MAPKAPK2 inhibitor PF-364402 (#PZ0188) and H2O2 (#H1009) from Sigma, AMZ30 (#539695) from Millipore, Z-VAD-FMK (#S8102) from Bio-Connect and necrostatin-1 (#T1547) from Tebubio.
Guffens L., Derua R, & Janssens V. (2023). PME-1 sensitizes glioblastoma cells to oxidative stress-induced cell death by attenuating PP2A-B55α-mediated inactivation of MAPKAPK2-RIPK1 signaling. Cell Death Discovery, 9, 265.
+ Open protocol
+ Expand
2

Evaluating MK2 Inhibitor in Murine Tumor Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
CT26 cell were purchased from ATCC (Manassas, VA). Cells were cultured in complete RPMI with 5% FBS, 1% penicillin/streptomycin, and 1% L-glutamine. CT26 cells were treated for 48 hours before injection with either 50uM of MK2 inhibitor PF364402 (Sigma Aldrich, St. Louis, MO) or DMSO vehicle control. Cells were injected at 105 in 50μl of PBS into the flank of 6–8 week old Balb/c mice. Some mice injected with cells exposed to MK2 inhibitors were treated with recombinant IL-1β, IL-6, and TNFα to replenish cytokine production in amounts of, 25 ng of IL-1β (Biolegend, San Diego, CA), 25 ng of TNFα (Biolegend), and 1 μg of IL-6 (Shenandoah, Warwick, PA). Treatments were administered 3 times/week from day 2 until day 13.
Ray A.L., Berggren K.L., Cruz S.R., Gan G.N, & Beswick E.J. (2017). Inhibition of MK2 suppresses IL-1β, IL-6, and TNF-α dependent colorectal cancer growth. International journal of cancer, 142(8), 1702-1711.
+ Open protocol
+ Expand
3

Investigating the Role of MK2 Inhibitor in Tumor Progression

Check if the same lab product or an alternative is used in the 5 most similar protocols
CT26 cells were purchased from ATCC (Manassas, VA, USA) and MC38 cells were obtained from NIH. Cells were cultured in complete RPMI with 10% FBS, 1% penicillin/streptomycin, and 1% l-glutamine. CT26 or MC38 cells were treated for 48 h before injection with either 50 μM of MK2 inhibitor PF364402 (Sigma Aldrich, St. Louis, MO, USA) or DMSO vehicle control. Cells were injected at 105 for CT26 and 2 × 106 for MC38 in 100 µl of PBS into the flank of 6- to 8-week-old Balb/c mice or C57/Bl6 mice. Some mice injected with cells exposed to MK2 inhibitors were injected intratumorally with recombinant MCP-1 (800 ng), Mip-1α (50 ng), and Mip-2α (700 ng) in 100 µl of PBS to replenish cytokine production or vehicle control. Treatments were administered 3 times/week from day 2 until day 13. Tumors were measured using calipers and divided for culture and RNA work.
Phinney B.B., Ray A.L., Peretti A.S., Jerman S.J., Grim C., Pinchuk I.V, & Beswick E.J. (2018). MK2 Regulates Macrophage Chemokine Activity and Recruitment to Promote Colon Tumor Growth. Frontiers in Immunology, 9, 1857.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!