Log In Sign up
The largest database of trusted experimental protocols

V450 anti mouse cd11b

Manufactured by BD
Visit Supplier
Sourced in United States

The V450-anti-mouse CD11b is a fluorochrome-conjugated antibody that binds to the CD11b antigen expressed on the surface of mouse myeloid cells. Its core function is to enable the detection and identification of CD11b-positive cells in various analytical and experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Mouse anti cd106 pe, by BD (2 mentions) Integrin β3, by Cell Signaling Technology (1 mentions) Gm csf, by BioLegend (1 mentions) Oscar, by R&D Systems (1 mentions) C fos, by Cell Signaling Technology (1 mentions) Recombinant mouse m csf, by BioLegend (1 mentions) Af 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Streptavidin apc cy7, by BioLegend (1 mentions) Rankl, by BioLegend (1 mentions) Interleukin 4 (il 4), by BioLegend (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Fc block, by BD (1 mentions) Facscalibur, by BD (1 mentions) Anti mouse cd45 fitc, by BD (1 mentions) Mouse anti cd90 pecytm7, by BD (1 mentions)

Close spelling variants of this product

Anti-CD11b-V450 CD11b-V450 Mouse anti-CD11b Anti-CD11b CD11b Mouse anti

4 protocols using v450 anti mouse cd11b

1

Osteoclastogenesis Regulation by Key Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant mouse M-CSF, GM-CSF, IL-4, and RANKL were purchased from BioLegend (San Diego, CA). AF488-phalloidin was purchased from Invitrogen (Carlsbad, CA). Antibodies for immunoblot assays were obtained against NFATc1 (Pierce, Rockford, IL); Oscar (R&D Systems, Minneapolis, MN); TRAP (BioLegend); Integrin β3; c-Fos; cleaved Caspase-3, -8, and -9; and cleaved PARP (Cell Signaling Technology, Mountain View, CA). The previously described anti-Ninj1 Ab1-1518 (link) was used for immunoblotting assays. For FACS analysis, Fc block, and PE-anti-mouse CD115, APC-anti-mouse CD117, and V450-anti-mouse CD11b antibodies were purchased from BD Biosciences (Bedford, MA), and biotin-anti-RANK antibody and APC/Cy7 streptavidin were obtained from BioLegend.
Bae S.J., Shin M.W., Son T., Lee H.S., Chae J.S., Jeon S., Oh G.T, & Kim K.W. (2019). Ninjurin1 positively regulates osteoclast development by enhancing the survival of prefusion osteoclasts. Experimental & Molecular Medicine, 51(1), 1-16.
+ Open protocol
+ Expand
2

Isolation and Quantification of Murine Bone Marrow Monocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow cells from female mice were harvested by flushing PBS through the bone cavity of the humerus using a syringe. After centrifugation, cells were resuspended in Tris-buffered 0.83% NH4Cl solution (pH 7.29) for 5 min to lyse erythrocytes and then washed in PBS. Bone marrow cells were resuspended in PBS supplemented with 10% FBS and 5 mM EDTA. Cells were stained with phycoerythrin (PE) anti-mouse Ly-6G (Gr-1) (12-5931-83, 1:50, eBioscience), V450 anti-mouse CD11b (560455, 1:50, BD Bioscience) and allophycocyanin (APC) anti-mouse CSF-1R (135510, 1:10, BioLegend). The cells were then subjected to FACS on a FACSCalibur (BD Pharmingen, Franklin Lakes, NJ) and analyzed using FlowJo software. The monocytes were gated from the side scatter (SSC) versus forward scatter (FSC). The pre-osteoclasts were defined as CSF-1R–positive, CD11b-positive and Gr1-low/negative monocytes and are presented as a cell frequency (%) of bone marrow cells.
Movérare-Skrtic S., Henning P., Liu X., Nagano K., Saito H., Börjesson A.E., Sjögren K., Windahl S.H., Farman H., Kindlund B., Engdahl C., Koskela A., Zhang F.P., Eriksson E.E., Zaman F., Hammarstedt A., Isaksson H., Bally M., Kassem A., Lindholm C., Sandberg O., Aspenberg P., Sävendahl L., Feng J.Q., Tuckermann J., Tuukkanen J., Poutanen M., Baron R., Lerner U.H., Gori F, & Ohlsson C. (2014). Osteoblast-derived WNT16 represses osteoclastogenesis and prevents cortical bone fragility fractures. Nature medicine, 20(11), 1279-1288.
+ Open protocol
+ Expand
3

Flow Cytometry Analysis of Second-Generation BMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
The density of second-generation BMSCs was adjusted to 2 × 107 cells/mL. The control group received 50 µL of buffer, while the single-label group received 5 µL of hamster anti-CD29-AF647 (BD Biosciences, Franklin Lakes, NJ, USA), mouse anti-CD90-PECyTM7 (BD Biosciences), mouse anti-CD106-PE (BD Biosciences), mouse anti-CD11b-V450 (BD Biosciences), or mouse anti-CD45-FITC (BD Biosciences) to each branch-flow sampling tube, after which 45 µL of buffer was added to each tube. The multicolor group received 5 µL of each antibody into a one-branch-flow sampling tube, and then, 25 µL of buffer was added. Next, 50 µL of cell suspension was added to each flow tube, the tubes were incubated at room temperature for 30 min and washed twice with staining buffer, and then, 500 µL of buffer was added to each tube for detection by flow cytometry (Beckman Coulter Life Sciences, Brea, CA, USA).
Zhang F., Peng W., Wang T., Zhang J., Dong W., Wang C., Xie Z., Luo H, & Liu G. (2022). Lnc Tmem235 promotes repair of early steroid-induced osteonecrosis of the femoral head by inhibiting hypoxia-induced apoptosis of BMSCs. Experimental & Molecular Medicine, 54(11), 1991-2006.
+ Open protocol
+ Expand
4

Multiparametric Flow Cytometry of Mouse Leukocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
First, 5 μL of mouse anti-CD90-PECyTM7, mouse anti-CD106-PE, mouse anti-CD11b-V450, and mouse anti-CD45-fluorescein isothiocyanate (FITC) (BD Biosciences, Franklin Lakes, NJ, USA) was added into an flow cytometry (FCM) tube, followed by the addition of 50 μL cell suspension (2 × 107/mL). The mixture was incubated at room temperature (RT) in the dark for 30 min, and the contents of each tube were then washed twice using a standing buffer. Then, 500 μL staining buffer was added to resuspend the cells, and FCM (Beckman Coulter Life Sciences, Brea, CA, USA) was used to detect expression levels of CD11b, CD45, CD90, and CD106.
Zhang F., Yan Y., Peng W., Wang L., Wang T., Xie Z., Luo H., Zhang J, & Dong W. (2021). PARK7 promotes repair in early steroid-induced osteonecrosis of the femoral head by enhancing resistance to stress-induced apoptosis in bone marrow mesenchymal stem cells via regulation of the Nrf2 signaling pathway. Cell Death & Disease, 12(10), 940.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!