Ho8910

Human ovarian carcinoma cell lines HO8910 and A2780 were obtained from the American Type Culture Collection (Manassas, VA). All cells were maintained in RPMI-1640 medium (Solarbio, Beijing, China) containing 10% fetal bovine serum (Thermo Fisher, Wilmington, DE, USA) and 1% penicillin-streptomycin solution (Procell, Wuhan, China), and cultured at 37 °C in 5% CO₂.
HO8910 and A2780 cells were dissociated with 0.5% trypsin and seeded into six-well plates. HO8910 and A2780 cells were infected with miR-600 knockdown virus and control virus. HO8910 and A2780 cells were infected with si-KLF9 and control siRNA. Then the stable infectants were screening by using puromycin as before [17 (link)]. miR-600 knockdown virus virus was obtained from Shanghai GenePharma (Shanghai, China). KLF9 siRNA was also obtained from Shanghai GenePharma (Shanghai, China).

Human ovarian cancer cell line A2780 (Procell, China, CL‐0013) and HO8910 (Procell, China, CL‐0113) were cultured in RPMI 1640 culture medium and SKOV3 (ATCC, USA, HBT‐77) in McCoy's 5A medium, supplemented with 10% fetal bovine serum (FBS, Gibco). OVCAR3 (ATCC, USA, HTB‐161) was cultured in RPMI1640 with 20% FBS (Gibco, USA). HEK293T (ATCC, USA, CRL‐3216) was cultured in DMEM culture medium with 10% FBS (Gibco, USA). All cells were grown at 37°C under 5% CO₂. Cisplatin was purchased from SIGMA ALDRICH (P4394, USA). RNA was extracted from each cell types three times, thus three relative gene expression levels of the target gene from each sample were obtained.

Four OC cell lines (OVCAR3, SKOV3, A2780, and HO-8910) and human ovarian immortalized nontumorigenic ovarian surface epithelial cells (IOSE) were acquired from Procell Life Science Co., Ltd.,China and cultured in Dulbecco’s modified Eagle medium (DMEM) (TransGen Biotech Co., Ltd., Beijing, China) containing 10% fetal bovine serum (Gibco Company, NY, USA) and 1% penicillin/streptomycin (Sigma-Aldrich Chemical Company., MO, USA). All the cells were incubated in humidified chambers [21 (link)].