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Maxtract high density phase separation tubes

Manufactured by Qiagen
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MaXtract High Density phase separation tubes are designed for rapid and efficient separation of aqueous and organic phases during nucleic acid extraction and purification processes. The tubes feature a high-density gel that effectively separates the phases, facilitating the recovery of the target analyte.

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Lab products found in correlation

Quantstudio thermo cycler, by Thermo Fisher Scientific (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) High capacity cdna kit, by Thermo Fisher Scientific (2 mentions) Sybr green master mix, by Thermo Fisher Scientific (1 mentions) Taqman master mix, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

MaXtract High Density tubes (32 citations) MaXtract High Density (14 citations) MaXtract tubes (10 citations)

2 protocols using maxtract high density phase separation tubes

1

RNA Extraction, cDNA Synthesis, and SYBR Green qPCR

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RNA was extracted using Trizol (Thermo Fisher Scientific 15596026) and MaXtract High Density phase separation tubes (Qiagen, 129056) per manufacturers’ protocols. RNA was DNAse-treated (Thermo Scientific, EN0525) and then cDNA was synthesized (Applied Biosystems High Capacity cDNA Kit, 4368814). The following SYBR Green qPCR primers were used in conjunction with SYBR Green Master Mix and the QuantStudio thermo cycler (Applied Biosystems), with Beta-Actin as a positive control:
Owens J.T., Miyake R., Murakami K., Chmura A.J., Fujita N., Ishihama A, & Meares C.F. (1998). Mapping the σ70 subunit contact sites on Escherichia coli RNA polymerase with a σ70-conjugated chemical protease. Proceedings of the National Academy of Sciences of the United States of America, 95(11), 6021-6026.
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2

Quantitative Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was extracted using Trizol (Thermo Fisher Scientific 15596026) and MaXtract High Density phase separation tubes (Qiagen, 129056) per manufacturers’ protocols. RNA was DNAse-treated (Thermo Scientific, EN0525) and then complementary DNA (cDNA) was synthesized (Applied Biosystems High Capacity cDNA Kit, 4368814). The following TaqMan qPCR primers were used in conjunction with TaqMan Master Mix and the QuantStudio thermo cycler (Applied Biosystems): murine Ifi27 (Mm00835449_g1), murine b-actin (Mm00607939_s1), human CCL5 (Hs99999048_m1), human ISG15 (Hs01921425_s1), human IFI27 (Hs01086373_g1), human ACTB (Hs01060665_g1).
Gomez S., Cox O.L., Walker RR I.I.I., Rentia U., Hadley M., Arthofer E., Diab N., Grundy E.E., Kanholm T., McDonald J.I., Kobyra J., Palmer E., Noonepalle S., Villagra A., Leitenberg D., Bollard C.M., Saunthararajah Y, & Chiappinelli K.B. (2022). Inhibiting DNA methylation and RNA editing upregulates immunogenic RNA to transform the tumor microenvironment and prolong survival in ovarian cancer. Journal for Immunotherapy of Cancer, 10(11), e004974.
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