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Purified water

Manufactured by Scharlab
Sourced in Spain

Purified water is a high-quality water product that has undergone a rigorous purification process to remove impurities, minerals, and contaminants. It is designed to meet the requirements of various laboratory applications where consistent, reliable, and pure water is essential.

Automatically generated - may contain errors

2 protocols using purified water

1

Lipid-Polymer Nanocarrier Formulation and Characterization

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DEX (Fagron Ibérica, Barcelona, Spain), ethyl cellulose (EC) (Ashaland Industries Europe GmbH, Rheinweg, Switzerland), Tween 80 and Span 60 (Croda Iberica S.A., Spain), benzalkonium chloride (Sigma Aldrich, Madrid, Spain), medium chain triglycerides (MCT) (Oxi-Med Expres S.A., Barcelona, Spain), ethanol absolute (ET) and ethyl acetate (EA) (Scharlab S.L., Barcelona, Spain), and purified water (Inhouse) were used to formulate the LPNCs. Coumarin 6 (C6) (Sigma Aldrich, St. Louis, MO, USA) and 1,2-dioleoyl-3-[16-N-(lissamine rhodamine B sulfonyl) amino]palmitoyl-sn-glycerol (LRB) (Avanti Polar Lipids, Alabaster, AL, USA) were used as fluorochromes. Paraformaldehyde (Scharlab S.L., Barcelona, Spain), Sucrose (Acor, Valladolid, Spain), optimal cutting temperature compound (OCT) (Tissue-Tek Sakura Finetek, Torrance, CA, USA), rabbit anti-DEX IgG (Abcam, Cambridge, UK), Alexa Fluor 488 goat anti rabbit (Life Technologies, Carlsbad, CA, USA), ProlongTM Gold Antifade mounting medium (Thermo Fisher Scientific, Barcelona, Spain), and Tween 20 (Sigma-Aldrich, St. Louis, MO, USA) were used to prepare samples for microscopic visualization.
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2

Transfersomes and Emulsion Formulation

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Ceramide III (Evonik Nutrition & Care, Essen, Germany), α-Tocopherol (Merck Chemicals and life, Barcelona, Spain), phosphatidylcholine (Lipoid, Ludwigshafen, Germany), Tween 80 (Croda Iberica S.A., Barcelona, Spain), Retynil Palmitate and Ethanol Absolute (Scharlab S.L. Barcelona, Spain) and purified water (Inhouse) were used to formulate the transfersomes. Dissodium EDTA (Sucesores de Jose Escuder, S.L., Barcelona, Spain), PEG-6 stearate (and) Ceteth-20 (and) Steareth-20 (Gattefosse España, Madrid, Spain), Cetyl Sterayl alcohol (Basf, Barcelona, Spain), medium chain triglicerides (Oximed expres S.A., Barcelona, Spain) and Xanthan gum (Azelis españa S.A., Barcelona, Spain) were used to formulate the emulsion. Metanol (Scharlab S.L., Barcelona, Spain), Nile Red, Hoeschst, phosphate buffer saline, paraformaldehyde (Sigma Aldrich, Madrid, Spain), uranyl acetate, optimal cutting temperature compound (IESMAT S.A., Barcelona, Spain), were used to perform the different analyses.
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